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Adv."],"published-print":{"date-parts":[[2026,1,16]]},"abstract":"<jats:p>\n                    Activation of virulence in\n                    <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                    , the etiological agent of cholera disease, is mediated by two transmembrane one-component signal-transduction proteins, ToxR and TcpP, which are also transcription factors. Using cryo\u2013electron microscopy, we have solved five structures of the\n                    <jats:italic toggle=\"yes\">ompU<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">toxT<\/jats:italic>\n                    transcription activation complexes, including the RNA polymerase (RNAP) holoenzyme, promoter DNAs, transcribed RNA, and their corresponding transcription factors, ToxR or TcpP and ToxR-TcpP, respectively. Activation is achieved through the interaction of ToxR or TcpP with the \u03b1\u2013C-terminal repeat domain of RNAP where a single residue of the activator, a phenylalanine, appears to be the most critical contact, as confirmed by mutagenesis. No interactions of the transcription factors were observed with other subunits of the RNAP, i.e., the \u03c3 subunit as it occurs in the structurally related PhoB family of two-component transcription factors. The structures, and their comparison with our previously solved DNA promoter\u2013ToxR x-ray structures, unveil the molecular mechanism of cholera virulence gene activation.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adx9680","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T18:59:36Z","timestamp":1768417176000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":5,"title":["Structures of\n                    <i>Vibrio cholerae<\/i>\n                    transcription complexes reveal how ToxR and TcpP recruit the RNA polymerase and activate virulence genes"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1015-6469","authenticated-orcid":true,"given":"Adri\u00e0","family":"Alcaide-Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), Barcelona Institute of Science and Technology (BIST), 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