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Adv."],"published-print":{"date-parts":[[2026,1,2]]},"abstract":"<jats:p>\n                    Mechanomodulation, the process of altering cellular behavior through applied mechanical forces, plays a critical role in physiological processes and has substantial implications for cancer therapy, immunology, and drug development. However, precise and efficient stimulation of nonadherent cells remains a major challenge, limiting the investigation of mechanotransduction pathways and the development of targeted therapeutics. Here, we developed an acoustofluidic platform named Suspension-cell Targeted Response to Excitation via Acoustofluidic Mechanomodulation (STREAM) to enable precise, high-throughput stimulation of suspension cells. STREAM accomplishes this using 101.14-megahertz high-frequency surface acoustic waves to deliver controlled mechanical stimulation at a throughput of 500,000 cells per minute. STREAM modulates intracellular calcium ion (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ) signaling by activating mechanosensitive ion channels, triggering mitochondrial membrane disruption and tunable K562 leukemia cell apoptosis rates from 5.15 to 47.1%. STREAM provides a scalable, precise tool for studying mechanotransduction in suspension cells, with broad applications in cancer research, immunotherapy, and high-throughput drug screening.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.ady1136","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T18:59:44Z","timestamp":1767380384000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":10,"title":["Precision acoustofluidics for high-throughput mechanobiology in suspension cells"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6666-4619","authenticated-orcid":true,"given":"Kaichun","family":"Yang","sequence":"first","affiliation":[{"name":"Thomas Lord Department of Mechanical Engineering &amp; Materials Science, Duke University, Durham, NC 27708, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0433-0923","authenticated-orcid":true,"given":"Ruoyu","family":"Zhong","sequence":"additional","affiliation":[{"name":"Thomas Lord Department of Mechanical Engineering &amp; Materials Science, Duke University, Durham, NC 27708, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0243-1811","authenticated-orcid":true,"given":"Ke","family":"Li","sequence":"additional","affiliation":[{"name":"Thomas Lord Department of Mechanical Engineering &amp; Materials Science, Duke University, Durham, NC 27708, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8001-9120","authenticated-orcid":true,"given":"John","family":"Mai","sequence":"additional","affiliation":[{"name":"Alfred E. 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