{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T22:10:40Z","timestamp":1785276640378,"version":"3.55.0"},"reference-count":67,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"47","funder":[{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["003140I221"],"award-info":[{"award-number":["003140I221"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008893","name":"University of Iowa","doi-asserted-by":"publisher","award":["Carver Faculty Start-Up Funds"],"award-info":[{"award-number":["Carver Faculty Start-Up Funds"]}],"id":[{"id":"10.13039\/100008893","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH","award":["R21 HL161499"],"award-info":[{"award-number":["R21 HL161499"]}]},{"name":"NIH","award":["R01 EB03339"],"award-info":[{"award-number":["R01 EB03339"]}]},{"name":"NIH","award":["R01 HL133089"],"award-info":[{"award-number":["R01 HL133089"]}]},{"name":"NIH","award":["R01 HL171035"],"award-info":[{"award-number":["R01 HL171035"]}]},{"name":"Parker B. Francis Fellowship"}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2025,11,21]]},"abstract":"<jats:p>\n                    Submucosal glands (SMGs) are critical for airway health; however, replicating their complex structure and function outside the body remains challenging. Here, we establish a method of airway tissue explant culture at a hydrogel-air interface (i.e., AirTECH) using trachea from newborn pigs. After 14 days, AirTECH tissues maintain their natural SMG structures, cell types, gene expression, and defense functions, along with intact surface epithelia, smooth muscle, and cartilage. Using AirTECH tissue as a model, we evaluated SMGs in cystic fibrosis (CF) airway disease progression, where exposure to lipopolysaccharide of\n                    <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                    caused abnormal mucus obstruction and overexpression of MUC5AC in SMGs, a characteristic feature of CF airway disease. We also explored the contribution of SMGs in airway gene therapy, where green fluorescent protein\u2013encoding adenovirus vectors were transduced and expressed exclusively in airway surface epithelia but not SMGs. These data support AirTECH as a model to understand SMGs in airway disease and test therapeutic interventions.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.ady4866","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T18:58:54Z","timestamp":1763578734000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":2,"title":["Porcine airway tissue explant culture at a hydrogel-air interface to model submucosal glands in cystic fibrosis progression and therapy"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7398-0466","authenticated-orcid":true,"given":"Kaleb C.","family":"Bierstedt","sequence":"first","affiliation":[{"name":"Roy J. Carver Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA, USA."},{"name":"Pappajohn Biomedical Institute, The University of Iowa, Iowa City, IA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2148-9760","authenticated-orcid":true,"given":"Meng-Hsuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Roy J. Carver Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA, USA."},{"name":"Pappajohn Biomedical Institute, The University of Iowa, Iowa City, IA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3013-1218","authenticated-orcid":true,"given":"Wenjie","family":"Yu","sequence":"additional","affiliation":[{"name":"Pappajohn Biomedical Institute, The University of Iowa, Iowa City, IA, USA."},{"name":"Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2717-9138","authenticated-orcid":true,"given":"Ashley L.","family":"Cooney","sequence":"additional","affiliation":[{"name":"Pappajohn Biomedical Institute, The University of Iowa, Iowa City, IA, USA."},{"name":"Stead Family Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Goodell","sequence":"additional","affiliation":[{"name":"Pappajohn Biomedical Institute, The University of Iowa, Iowa City, IA, USA."},{"name":"Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyu","family":"Gong","sequence":"additional","affiliation":[{"name":"Pappajohn Biomedical Institute, The University of Iowa, Iowa City, IA, USA."},{"name":"Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7544-5109","authenticated-orcid":true,"given":"Alejandro A.","family":"Pezzulo","sequence":"additional","affiliation":[{"name":"Pappajohn Biomedical Institute, The University of Iowa, Iowa City, IA, USA."},{"name":"Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9746-966X","authenticated-orcid":true,"given":"Patrick L.","family":"Sinn","sequence":"additional","affiliation":[{"name":"Pappajohn Biomedical Institute, The University of Iowa, Iowa City, IA, USA."},{"name":"Stead Family Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6084-7113","authenticated-orcid":true,"given":"Yuliang","family":"Xie","sequence":"additional","affiliation":[{"name":"Roy J. 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