{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:46:43Z","timestamp":1790351203968,"version":"4.1.0"},"reference-count":107,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"38","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2025,9,19]]},"abstract":"<jats:p>\n                    Gangliosides are glycosphingolipids with important roles in cell signaling and neuroprotection. While present on extracellular vesicles (EVs)\u2014key mediators of intercellular communication\u2014their role in EV biogenesis remains unclear. Here, we identify gangliosides as key modulators of EV biogenesis, with the specific composition of their glycan headgroup and the presence or absence of sialic acid and\n                    <jats:italic toggle=\"yes\">N<\/jats:italic>\n                    -acetyl-\n                    <jats:sc>d<\/jats:sc>\n                    -galactosamine residues dictating whether they promote or inhibit EV biogenesis. GM1 and other complex gangliosides enhance EV secretion, while disruption of ganglioside synthesis impairs it. GM1 supplementation restores EV secretion in Huntington\u2019s disease (HD) fibroblasts and cell models with ganglioside deficiency, including models of neurodegenerative diseases caused by a genetic block of ganglioside synthesis. Notably, GM1 enhances EV-mediated secretion of pathogenic misfolded proteins, including mutant huntingtin (mHTT), \u03b1-synuclein, and tau, reducing intracellular burden and providing mechanistic insight into the mHTT-lowering effects of GM1 in HD models. Our findings shed light on the neuroprotective roles of gangliosides and their therapeutic potential in misfolded protein disorders.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.ady5212","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T17:59:22Z","timestamp":1758131962000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":11,"title":["Gangliosides modulate the secretion of extracellular vesicles and their misfolded protein cargo"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6909-9147","authenticated-orcid":true,"given":"John","family":"Monyror","sequence":"first","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Glycomics Institute of Alberta, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8530-1472","authenticated-orcid":true,"given":"Vaibhavi","family":"Kadam","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4194-2961","authenticated-orcid":true,"given":"Luis Carlos","family":"Morales","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7693-1625","authenticated-orcid":true,"given":"Diego","family":"Ord\u00f3\u00f1ez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0829-9516","authenticated-orcid":true,"given":"Jeremies","family":"Ibanga","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Glycomics Institute of Alberta, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4515-1390","authenticated-orcid":true,"given":"Asifa K.","family":"Zaidi","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Glycomics Institute of Alberta, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"McNamara","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Deparment of Biochemistry, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7543-3199","authenticated-orcid":true,"given":"Desmond","family":"Pink","sequence":"additional","affiliation":[{"name":"Department of Oncology, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Nanostics Inc., Edmonton, Alberta T6N1H1, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Stenlund","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2416-6723","authenticated-orcid":true,"given":"Ken","family":"Reyes","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Glycomics Institute of Alberta, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4622-4598","authenticated-orcid":true,"given":"Aislinn D.","family":"Maguire","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4080-8728","authenticated-orcid":true,"given":"Leonardo M.","family":"Cortez","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Centre for Prions and Protein Folding Diseases, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-8666","authenticated-orcid":true,"given":"Valerie L.","family":"Sim","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Centre for Prions and Protein Folding Diseases, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Medicine, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4593-3368","authenticated-orcid":true,"given":"Sue-Ann","family":"Mok","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Deparment of Biochemistry, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0493-7282","authenticated-orcid":true,"given":"Elena","family":"Posse de Chaves","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3060-0723","authenticated-orcid":true,"given":"Simonetta","family":"Sipione","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Glycomics Institute of Alberta, University of Alberta, Edmonton, AB T6G2R3, Canada."},{"name":"Department of Pharmacology, University of Alberta, Edmonton, AB T6G2R3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.125"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0759-6_10"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvaa131"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40680-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2015.29"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2021.102124"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep07989"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aww237"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.10.019"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25855-2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308413101"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-016-0143-y"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-018-1868-1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2017.00149"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2021.788695"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2023.108486"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-024-03060-4"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-019-1532-2"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.E086173"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.722581"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12319"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1714085115"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M114.047530"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.4.1141"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3510607"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1736"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-03-0871"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R084210"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.572965"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.accb.2018.09.002"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-022-10052-0"},{"key":"e_1_3_2_33_2","first-page":"177","article-title":"Lipid rafts in exosome biogenesis","volume":"85","author":"Skryabin G. O.","year":"2020","unstructured":"G. O. Skryabin, A. V. Komelkov, E. E. Savelyeva, E. M. Tchevkina, Lipid rafts in exosome biogenesis. Biochemistry 85, 177\u2013191 (2020).","journal-title":"Biochemistry"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwl052"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1722320115"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v5.32570"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2007.05.003"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-019-0339-z"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1177\/1759091418781889"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.06964.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6348-09.2010"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2013.05.006"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awt270"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt434"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1460"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201707763"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1114502109"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42847-x"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2012.10.024"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2021.06.005"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00964917"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.09.018"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1587567"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells11030351"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03071-y"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461773"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24384-2"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1521230113"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v2i0.19671"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1896-7_17"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12079"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23158544"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep37246"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.2337\/db06-0719"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2016.00457"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1159\/000213109"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23090"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011027125744"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38030-6"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01115-6"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2628045100"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.19.2799"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2017.12.004"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9091955"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.837930"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15747-2"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.08.002"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1153124"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2018.01.002"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R083915"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2017.10.022"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.25.15253"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c03196"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107756200"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1149-14.2014"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells2040751"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703650104"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.12.010"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1016\/0197-0186(92)90057-X"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1418-17.2017"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.1002\/jex2.65"},{"key":"e_1_3_2_92_2","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1560809"},{"key":"e_1_3_2_93_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00403"},{"key":"e_1_3_2_94_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.04.005"},{"key":"e_1_3_2_95_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.762439"},{"key":"e_1_3_2_96_2","doi-asserted-by":"crossref","unstructured":"T. Isogai K. M. Hirosawa M. Kanno A. Sho R. S. Kasai N. Komura H. Ando K. Furukawa Y. Ohmi K. Furukawa Y. Yokota K. G. N. Suzuki Extracellular vesicles adhere to cells predominantly through the interaction of CD151-associated integrin heterodimers and GM1 with laminin. bioRxiv 2024.04.11.589011 [Preprint] (2024); https:\/\/doi.org\/10.1101\/2024.04.11.589011.","DOI":"10.1101\/2024.04.11.589011"},{"key":"e_1_3_2_97_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02374-x"},{"key":"e_1_3_2_98_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-023-02963-y"},{"key":"e_1_3_2_99_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-0965-7"},{"key":"e_1_3_2_100_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.01.013"},{"key":"e_1_3_2_101_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-2756"},{"key":"e_1_3_2_102_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205514"},{"key":"e_1_3_2_103_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490350513"},{"key":"e_1_3_2_104_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024249707516"},{"key":"e_1_3_2_105_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2017.08.019"},{"key":"e_1_3_2_106_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-021-01132-y"},{"key":"e_1_3_2_107_2","first-page":"Unit 3.22","article-title":"Isolation and characterization of exosomes from cell culture supernatants and biological fluids","volume":"3","author":"Thery C.","year":"2006","unstructured":"C. Thery, S. Amigorena, G. Raposo, A. Clayton, Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr. Protoc. Cell Biol. 3, Unit 3.22 (2006).","journal-title":"Curr. Protoc. Cell Biol."},{"key":"e_1_3_2_108_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024406221962"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.ady5212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T18:01:13Z","timestamp":1758132073000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.ady5212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,19]]},"references-count":107,"journal-issue":{"issue":"38","published-print":{"date-parts":[[2025,9,19]]}},"alternative-id":["10.1126\/sciadv.ady5212"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.ady5212","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.04.03.647108","asserted-by":"object"}]},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,19]]},"assertion":[{"value":"2025-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eady5212"}}