{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T19:27:22Z","timestamp":1791833242273,"version":"4.3.4"},"reference-count":62,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"1","funder":[{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","award":["NE\/T000848\/1"],"award-info":[{"award-number":["NE\/T000848\/1"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","award":["NE\/X009742\/1"],"award-info":[{"award-number":["NE\/X009742\/1"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Human Frontiers","award":["RGP007\/2024"],"award-info":[{"award-number":["RGP007\/2024"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,1,2]]},"abstract":"<jats:p>\n                    Diatoms use multiple mechanisms of dissolved inorganic carbon (DIC) uptake to maintain CO\n                    <jats:sub>2<\/jats:sub>\n                    supply to RuBisCO (ribulose-1,5-bisphosphate carboxylase\/oxygenase), but how these different pathways are coordinated in response to rapid changes in their environment remains unclear. By measuring the dynamic changes in phycosphere carbonate chemistry around single diatom cells, we show that DIC uptake is rapidly modulated. We find that external carbonic anhydrase (eCA) plays a major role in DIC uptake in large centric diatoms at ambient seawater CO\n                    <jats:sub>2<\/jats:sub>\n                    , but there is a strong switch to direct HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    uptake under low CO\n                    <jats:sub>2<\/jats:sub>\n                    . Inhibition of eCA leads to rapid activation of HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    uptake, which can compensate for the loss of eCA activity under lower irradiances. These different modes of DIC uptake have distinct influences on phycosphere carbonate chemistry that may directly affect nutrient acquisition and biotic interactions. Our findings reveal that dynamic modulation of DIC uptake allows diatoms to rapidly respond to changes in CO\n                    <jats:sub>2<\/jats:sub>\n                    supply and demand.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.ady8646","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T18:59:44Z","timestamp":1767380384000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":6,"title":["Dynamic changes in phycosphere carbonate chemistry reveal rapid modulation of carbon uptake in single diatom cells"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7609-3407","authenticated-orcid":true,"given":"Matthew","family":"Keys","sequence":"first","affiliation":[{"name":"The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Highfield","sequence":"additional","affiliation":[{"name":"The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6223-6049","authenticated-orcid":true,"given":"Abdul","family":"Chrachri","sequence":"additional","affiliation":[{"name":"The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6923-5535","authenticated-orcid":true,"given":"Clare","family":"Ostle","sequence":"additional","affiliation":[{"name":"The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7838-230X","authenticated-orcid":true,"given":"Colin","family":"Brownlee","sequence":"additional","affiliation":[{"name":"The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, UK."},{"name":"School of Ocean and Earth Sciences, University of Southampton, Southampton SO14 3ZH, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4657-1701","authenticated-orcid":true,"given":"Glen L.","family":"Wheeler","sequence":"additional","affiliation":[{"name":"The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1509523113"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-marine-120709-142720"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nclimate1696"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4203(97)00069-8"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2016.0403"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1216234110"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erac380"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2006.51.5.2111"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2003.48.1.0055"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx102"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jembe.2009.05.017"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.2008.01054.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.12572"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.csr.2012.04.012"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-8137.1997.00625.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nclimate2683"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s101520000064"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(99)01504-6"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12829"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.217737"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13728"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2007.5.328"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-007-9221-x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02426-y"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/plankt\/fbp127"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.13416"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2022030289"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.0000000000000008"},{"key":"e_1_3_2_30_2","first-page":"35","article-title":"The carbonate system of the North Sea: Determination of alkalinity and TCO2 and calculation of PCO2 and Slcal (spring 1986)","volume":"65","author":"Pegler K.","year":"1988","unstructured":"K. Pegler, S. Kempe, The carbonate system of the North Sea: Determination of alkalinity and TCO2 and calculation of PCO2 and Slcal (spring 1986). Mitt. Geol.Pal\u00e4ont. Inst. Univ. Hamburg. 65, 35\u201387 (1988).","journal-title":"Mitt. Geol.Pal\u00e4ont. Inst. Univ. Hamburg."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.12269"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-017-0476-6"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4203(03)00033-1"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-022-01280-1"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature25982"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa575"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.12.004"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/pld3.472"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.2007.01038.x"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2001.46.6.1378"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3040.1998.00321.x"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04662-9"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19328"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx173"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae463"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.77.2.253"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687860400014872"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/lno.12331"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pocean.2005.02.008"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.5194\/bg-11-2503-2014"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1071\/FP13247"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.5670\/oceanog.2015.33"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2014.59.3.1027"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1018062108"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055562"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2021.112008"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.5194\/bg-19-295-2022"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028983"},{"key":"e_1_3_2_59_2","unstructured":"H. S. Findlay A. Beesley J. Nunes; I. J. Brown V. Kitidis J. Fishwick T. Smyth \u201cInorganic carbon and total alkalinity at the Western channel observatory from the L4 site from 2008 to 2022\u201d (NERC EDS British Oceanographic Data Centre NOC 2023)."},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2008.6.532"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10872-014-0243-7"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1981.26.4.0717"},{"key":"e_1_3_2_63_2","doi-asserted-by":"crossref","unstructured":"R. Zeebe D. Wolf-Gladrow CO2 in Seawater: Equilibrium Kinetics Isotopes Elsevier Oceanography Book Series (Elsevier 2001) vol. 65 pp. 1\u2013346.","DOI":"10.1016\/S0422-9894(01)80002-7"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.ady8646","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T19:01:46Z","timestamp":1767380506000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.ady8646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,2]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,2]]}},"alternative-id":["10.1126\/sciadv.ady8646"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.ady8646","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,2]]},"assertion":[{"value":"2025-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eady8646"}}