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Adv."],"published-print":{"date-parts":[[2026,4,10]]},"abstract":"<jats:p>\n                    B cells are crucial for adaptive immunity, orchestrating humoral responses by producing antibodies essential for pathogen clearance. Here, we show that Poly(rC) binding protein 1 (\n                    <jats:italic toggle=\"yes\">Pcbp1<\/jats:italic>\n                    ), a multifunctional RNA binding protein, is a key regulator of antibody production in B cells.\n                    <jats:italic toggle=\"yes\">Pcbp1<\/jats:italic>\n                    deficiency in B cells resulted in significant reductions in immunoglobulin M expression at steady state and compromised differentiation of germinal center B cells and production of high-affinity antibodies upon immunization. These effects were caused by defective mitochondrial integrity in\n                    <jats:italic toggle=\"yes\">Pcbp1<\/jats:italic>\n                    -deficient B cells, including impaired mitochondrial electron transport chain complex I and elevated mitochondrial reactive oxygen species production. Mechanistically,\n                    <jats:italic toggle=\"yes\">Pcbp1<\/jats:italic>\n                    binds to the 3\u2032 untranslated region of\n                    <jats:italic toggle=\"yes\">Fdxr<\/jats:italic>\n                    messenger RNA to promote its expression, thereby supporting iron-sulfur cluster biogenesis, the assembly of mitochondrial complex I, and other Fdxr-dependent processes. Our findings reveal a previously unidentified role for\n                    <jats:italic toggle=\"yes\">Pcbp1<\/jats:italic>\n                    in regulating mitochondrial function, protein synthesis, and antibody responses in B cells, providing insight into posttranscriptional regulation and mitochondrial functions in adaptive immunity.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adz9095","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T18:01:06Z","timestamp":1775844066000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["RNA binding protein\n                    <i>Pcbp1<\/i>\n                    maintains mitochondria integrity to promote antibody production and germinal center response"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6360-9064","authenticated-orcid":true,"given":"Lizhen","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8854-1316","authenticated-orcid":true,"given":"Li","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."},{"name":"Fudan University, 220 Handan Road, Shanghai 200433, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9170-9054","authenticated-orcid":true,"given":"Tuan","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."},{"name":"Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China."},{"name":"Center for Genome Editing, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6478-2732","authenticated-orcid":true,"given":"Geng","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixing","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."},{"name":"Fudan University, 220 Handan Road, Shanghai 200433, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiancheng","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4915-6260","authenticated-orcid":true,"given":"Yifan","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4716-5115","authenticated-orcid":true,"given":"Yue","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5072-9225","authenticated-orcid":true,"given":"Xing","family":"Chang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Gene Expression, School of Medicine, Westlake University, Hangzhou, Zhejiang, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China."},{"name":"Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China."},{"name":"Center for Genome Editing, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China."},{"name":"Research Center for Industries of the Future, Westlake University, Hangzhou, Zhejiang, China."},{"name":"Affiliated Hangzhou First People\u2019s Hospital, School of Medicine, Westlake University, Hangzhou, Zhejiang, 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