{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:26:28Z","timestamp":1792787188014,"version":"4.5.0"},"reference-count":67,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"8","funder":[{"name":"Science and Technology Program of Guangdong Province in China","award":["2017B030301016"],"award-info":[{"award-number":["2017B030301016"]}]},{"name":"Shenzhen University 2035 Program for Excellent Research","award":["2024B004"],"award-info":[{"award-number":["2024B004"]}],"id":[{"id":"https:\/\/ror.org\/01vy4gh70","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Scientific Major Research Project of Anhui Higher Education Institutions","award":["2024AH040244"],"award-info":[{"award-number":["2024AH040244"]}]},{"name":"Anhui Provincial Clinical Medicine Research Transformation Special Project","award":["202427b10020034"],"award-info":[{"award-number":["202427b10020034"]}]},{"name":"Key Project of Anhui Province University Outstanding Young Talents Support Fund","award":["gzyqZD2021143"],"award-info":[{"award-number":["gzyqZD2021143"]}]},{"name":"Development of Modern Medical and Pharmaceutical Industry in Anhui Province project","award":["2021\/2022"],"award-info":[{"award-number":["2021\/2022"]}]},{"name":"Outstanding Youth Project of Anhui University natural science","award":["2023AH020050"],"award-info":[{"award-number":["2023AH020050"]}],"id":[{"id":"https:\/\/ror.org\/05th6yx34","id-type":"ROR","asserted-by":"crossref"}]},{"name":"2023 Anhui Provincial Health and Health Research Project","award":["AHWJ2023Bba20065"],"award-info":[{"award-number":["AHWJ2023Bba20065"]}]},{"name":"the Program for Youzuzhikeyan of Shenzhen University","award":["SZU2024YZZKY002"],"award-info":[{"award-number":["SZU2024YZZKY002"]}],"id":[{"id":"https:\/\/ror.org\/01vy4gh70","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,2,20]]},"abstract":"<jats:p>\n                    Despite advances in chimeric antigen receptor T cell (CAR T cell) therapy for leukemia and lymphoma, solid tumors remain challenging because of limited target specificity and safety concerns. Neoantigens like KRAS\n                    <jats:sup>G12V<\/jats:sup>\n                    , a highly prevalent yet undruggable mutation in solid tumors, offer tumor-exclusive specificity. This study developed CAR T cells targeting KRAS\n                    <jats:sup>G12V<\/jats:sup>\n                    \/HLA-A*02:01 using phage antibody display to identify high-affinity single-chain variable fragments. Engineered B9 CAR T cells specifically lysed tumor cells and patient-derived cancer organoids expressing KRAS\n                    <jats:sup>G12V<\/jats:sup>\n                    \/HLA-A*02:01, demonstrating potent antitumor activity. Animal studies showed that B9 CAR T cells effectively controlled tumor growth in subcutaneous pancreatic ductal adenocarcinoma (PDAC) xenografts, as well as in metastatic and peritoneal PDAC models. Safety assessments in NCG-HLA-A2.1 and C57BL\/6 mice revealed no detectable in vivo toxicity, supporting the clinical applicability of B9 CAR T cells. Collectively, our neoantigen-targeted CAR T cell therapy against solid tumors shows great potential for future clinical trials in patients with KRAS\n                    <jats:sup>G12V<\/jats:sup>\n                    \/HLA-A*02:01, paving the way for clinical translation.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aea2511","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T18:58:58Z","timestamp":1771613938000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":2,"title":["KRAS\n                    <sup>G12V<\/sup>\n                    \/HLA-A*02:01\u2013targeted chimeric antigen receptor T cells exhibit potent preclinical activity against solid tumors"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1846-6805","authenticated-orcid":true,"given":"Huimin","family":"Shao","sequence":"first","affiliation":[{"name":"Precision Medicine Centre, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8906-1964","authenticated-orcid":true,"given":"Fei","family":"Xu","sequence":"additional","affiliation":[{"name":"Precision Medicine Centre, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangyue","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Thyroid Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingjie","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Wannan Medical College, Wuhu 241002, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Wu","sequence":"additional","affiliation":[{"name":"Precision Medicine Centre, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianjun","family":"He","sequence":"additional","affiliation":[{"name":"Precision Medicine Centre, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyi","family":"Qian","sequence":"additional","affiliation":[{"name":"Precision Medicine Centre, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijie","family":"He","sequence":"additional","affiliation":[{"name":"Precision Medicine Centre, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanlin","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yabin","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoxiang","family":"Luo","sequence":"additional","affiliation":[{"name":"Wuhan Proligand Biotechnology Co. Ltd., Wuhan 430075, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2643-6278","authenticated-orcid":true,"given":"Li","family":"Fu","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Genome Stability and Disease Prevention, Department of Pharmacology and Shenzhen International Cancer Center, Shenzhen University Medical School, Shenzhen 518060, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7089-6880","authenticated-orcid":true,"given":"Zhenyu","family":"Xu","sequence":"additional","affiliation":[{"name":"Precision Medicine Centre, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, Wuhu 241001, China."},{"name":"Anhui Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern, Wuhu 241002, China."},{"name":"Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wannan Medical College, Wuhu 241002, China."},{"name":"Anhui Innovative Center for Drug Basic Research of Metabolic Diseases, Wuhu 241002, China."},{"name":"Anhui Province Key Laboratory of Non-coding RNA Basic and Clinical Transformation, Wuhu 241002, China."},{"name":"Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institutes, Wannan Medical College, Wuhu 241002, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21708"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0421-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-020-01001-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.34213"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2019.07.015"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2017.0184"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2019.01.002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-022-00704-3"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.672356"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-15-0188"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2015-0084"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2023.102699"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112979"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-4523-7"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-019-03344-9"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-022-00671-9"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42010-1"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI184782"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2025.05.006"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010337"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-021-00210-y"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02600-13"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2011.52"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-01-545020"},{"key":"e_1_3_2_26_2","first-page":"311","article-title":"Flow cytometric screening of yeast surface display libraries","volume":"263","author":"Feldhaus M.","year":"2014","unstructured":"M. Feldhaus, R. Siegel, Flow cytometric screening of yeast surface display libraries. Methods Mol. Biol. 263, 311\u2013332 (2014).","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21239163"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07762-w"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2024-010540"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn5647"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38064-w"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3006034"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-021-00189-2"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00176"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07205-6"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1917239"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.23.00434"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1694-1"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-24-0027"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.181873"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1158\/2767-9764.CRC-21-0165"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.17305\/bb.2024.10431"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.09.044"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06123-3"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-23-2098"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-21-1331"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-44342-4"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40364-023-00534-0"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2021-004035"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05531-1"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202451079"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI99538"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf2288"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-023-01492-8"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103250"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14051325"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2015.11.004"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-071114-040733"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2887"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.11.013"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005661"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2015.1049803"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1700236"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abb5191"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37212-3"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-1300"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2859"}],"updated-by":[{"DOI":"10.1126\/sciadv.aem1185","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000}}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aea2511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:58:35Z","timestamp":1789581515000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aea2511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,20]]},"references-count":67,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,2,20]]}},"alternative-id":["10.1126\/sciadv.aea2511"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aea2511","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,20]]},"assertion":[{"value":"2025-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaea2511"}}