{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:20:59Z","timestamp":1792632059972,"version":"4.5.0"},"reference-count":49,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"23","funder":[{"name":"Institute Strategic Programme: Advancing Plant Health (APH) Partner gran","award":["BB\/Y002997\/1"],"award-info":[{"award-number":["BB\/Y002997\/1"]}]},{"name":"PIKOBODIES: Made-to-order plant disease resistance genes using receptor-nanobody fusions","award":["EP\/Y032187\/1"],"award-info":[{"award-number":["EP\/Y032187\/1"]}]},{"name":"Mechanisms of pathogen suppression of NLR-mediated immunity","award":["BB\/V002937\/1"],"award-info":[{"award-number":["BB\/V002937\/1"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,6,5]]},"abstract":"<jats:p>\n                    Helper NLRs function as central nodes in plant immune networks. Upon activation, they oligomerize into inflammasome-like resistosomes to initiate immune signaling, yet the dynamics of resistosome assembly remain poorly understood. Here, we show that the virulence effector AVRcap1b from the Irish potato famine pathogen\n                    <jats:italic toggle=\"yes\">Phytophthora infestans<\/jats:italic>\n                    suppresses immune activation by directly engaging oligomerization intermediates of the tomato helper NLR SlNRC3. Cryo-EM structures of SlNRC3 in AVRcap1b-bound and unbound states reveal that AVRcap1b bridges multiple protomers, stabilizing a stalled intermediate and preventing formation of a functional resistosome. Leveraging AVRcap1b as a molecular tool, we also capture an additional SlNRC3 resistosome intermediate showing that assembly proceeds in a stepwise manner from dissociated monomers. These findings uncover a previously unrecognized vulnerability in NLR activation and reveal a pathogen strategy that disrupts immune complex assembly. This work advances mechanistic understanding of resistosome formation and uncovers a previously unrecognized facet of pathogen-plant coevolution.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeb1931","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T17:59:36Z","timestamp":1780682376000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":4,"title":["A plant pathogen effector blocks stepwise assembly of a helper NLR resistosome"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3161-1849","authenticated-orcid":true,"given":"Benjamin A.","family":"Seager","sequence":"first","affiliation":[{"name":"The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7636-4917","authenticated-orcid":true,"given":"Adeline","family":"Harant","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6002-0730","authenticated-orcid":true,"given":"Mauricio P.","family":"Contreras","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8434-5545","authenticated-orcid":true,"given":"Liang-Yu","family":"Hou","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1616-1872","authenticated-orcid":true,"given":"Chih Hang","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0290-0315","authenticated-orcid":true,"given":"Sophien","family":"Kamoun","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6491-0423","authenticated-orcid":true,"given":"Jogi","family":"Madhuprakash","sequence":"additional","affiliation":[{"name":"The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK."},{"name":"Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2812"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-023-00849-x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.03.012"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-080620-104948"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat2623"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202357495"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav5870"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adr2594"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac5489"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac5789"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.272278.115"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.03.017"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1702041114"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.108.095034"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.107201"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08521-7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav5868"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07668-7"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002868"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001136"},{"key":"e_1_3_2_22_2","first-page":"eadi9613","article-title":"Resurrection of plant disease resistance proteins via helper NLR bioengineering","volume":"9","author":"Contreras M. 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