{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:19:43Z","timestamp":1773879583719,"version":"3.50.1"},"reference-count":64,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"11","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32300712"],"award-info":[{"award-number":["32300712"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82401952"],"award-info":[{"award-number":["82401952"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32571306"],"award-info":[{"award-number":["32571306"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32230028"],"award-info":[{"award-number":["32230028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Research and Development Program of China","award":["2024YFC2706604"],"award-info":[{"award-number":["2024YFC2706604"]}]},{"name":"High Level Talents Introduction Funding of Guangdong Second Provincial General Hospital","award":["YY2022-005"],"award-info":[{"award-number":["YY2022-005"]}]},{"name":"Key Basic and Applied Research Project of Guangdong Province","award":["2023B1515120027"],"award-info":[{"award-number":["2023B1515120027"]}]},{"name":"Young Elite Scientists Sponsorship Program by China Association for Science and Technology","award":["YESS20240434"],"award-info":[{"award-number":["YESS20240434"]}]},{"name":"Science and Technology Projects in Guangzhou","award":["2023A03J0258"],"award-info":[{"award-number":["2023A03J0258"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,3,13]]},"abstract":"<jats:p>The spindle assembly checkpoint (SAC) is vital for preventing oocyte aneuploidy, a leading cause of female infertility, miscarriages, and trisomy syndromes. However, whether deubiquitination participates in SAC regulation remains unknown. Here, we reveal that the deubiquitinase USP8 acts as a SAC regulator to prevent aneuploid egg formation. Mechanistically, depletion of USP8 inactivates the SAC, accelerates meiotic progression, and causes abnormal spindle assembly and chromosome alignment, ultimately leading to aneuploidy. Intriguingly, we identify USP8 in oocytes as a previously unidentified interaction partner of BUB3, a key component of the SAC, and demonstrate that USP8 stabilizes BUB3 through its deubiquitinating activity. Moreover, exogenous BUB3 rescues the defects observed in USP8-depleted oocytes. Together, our findings not only clarify that deubiquitination participates in regulating the SAC in oocytes but also uncover a unique role for USP8 in controlling the SAC via its interaction with BUB3.<\/jats:p>","DOI":"10.1126\/sciadv.aeb2345","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:58:33Z","timestamp":1773424713000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Deubiquitinase USP8 regulates the spindle assembly checkpoint in oocytes"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6271-4231","authenticated-orcid":true,"given":"Changyin","family":"Zhou (\u5468\u957f\u94f6)","sequence":"first","affiliation":[{"name":"Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism &amp; Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou 510317, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9015-0517","authenticated-orcid":true,"given":"Xue","family":"Zhang (\u5f20\u96ea)","sequence":"additional","affiliation":[{"name":"Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism &amp; Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou 510317, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9890-2206","authenticated-orcid":true,"given":"Genlu","family":"Xu (\u8bb8\u6839\u9732)","sequence":"additional","affiliation":[{"name":"Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism &amp; Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou 510317, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Wang (\u738b\u6167)","sequence":"additional","affiliation":[{"name":"Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism &amp; Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou 510317, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuting","family":"Ran (\u5189\u5b87\u5a77)","sequence":"additional","affiliation":[{"name":"Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism &amp; Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou 510317, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ang","family":"Li (\u674e\u6602)","sequence":"additional","affiliation":[{"name":"Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism &amp; Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou 510317, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0148-2414","authenticated-orcid":true,"given":"Qing-Yuan","family":"Sun (\u5b59\u9752\u539f)","sequence":"additional","affiliation":[{"name":"Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism &amp; Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou 510317, China."},{"name":"Key Laboratory of Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China."},{"name":"Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9047-1612","authenticated-orcid":true,"given":"Xiang-Hong","family":"Ou (\u6b27\u6e58\u7ea2)","sequence":"additional","affiliation":[{"name":"Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism &amp; Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou 510317, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-021-00365-x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.152"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2731"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-061516-044916"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3982"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.10.027"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abg2653"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adq9111"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.200584.112"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.96353.4"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01566-06"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.081679"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-14-0671"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.030866"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114248"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3230"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"e03865","DOI":"10.1002\/advs.202503865","article-title":"USP8-governed MDA5 homeostasis promotes innate immunity and autoimmunity","volume":"12","author":"Zhang Q.","year":"2025","unstructured":"Q. Zhang, S. Huang, Y. He, W. Wang, C. Tong, M. Ma, M. Zhao, L. Yi, K. P. Knobeloch, P. Zhang, USP8-governed MDA5 homeostasis promotes innate immunity and autoimmunity. Adv. Sci. 12, e03865 (2025).","journal-title":"Adv. Sci."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1523597113"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2315541121"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202302953"},{"key":"e_1_3_2_22_2","first-page":"230","article-title":"Suppression of USP8 sensitizes cells to ferroptosis via SQSTM1\/p62-mediated ferritinophagy","volume":"14","author":"Liu L.","year":"2023","unstructured":"L. Liu, B. Zheng, M. Luo, J. Du, F. Yang, C. Huang, Z. Ma, C. Li, D. Guo, H. Peng, Suppression of USP8 sensitizes cells to ferroptosis via SQSTM1\/p62-mediated ferritinophagy. Protein Cell 14, 230\u2013234 (2023).","journal-title":"Protein Cell"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05747-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29401-6"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-022-01102-z"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021108791"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0419-1"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-3696"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-3979"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-025-07736-4"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41698-025-00850-8"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2022.01.015"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04749-1"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.215"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3166"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.20"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noz109"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1418335112"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.02.033"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001238"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201489729"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09430-4"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1635381"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2024.2395134"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00517-3"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.090589"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00593-z"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-010818-021649"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2017.00109"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.06.069"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175326"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202413097"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200102093"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.10.033"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-018-0028-3"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/s10038-023-01209-2"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2020.04.003"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00083-7"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.12.073"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg9335"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.00117"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7946"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.01.064"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw463"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aax3969"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aeb2345","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T18:00:10Z","timestamp":1773856810000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aeb2345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,13]]},"references-count":64,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,3,13]]}},"alternative-id":["10.1126\/sciadv.aeb2345"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aeb2345","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,13]]},"assertion":[{"value":"2025-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaeb2345"}}