{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T03:11:29Z","timestamp":1789614689857,"version":"build-2803163510"},"reference-count":44,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"12","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52425304"],"award-info":[{"award-number":["52425304"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20156"],"award-info":[{"award-number":["U22A20156"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515011130"],"award-info":[{"award-number":["2024A1515011130"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangzhou Key Research and Development-Program","award":["2025B03J0158"],"award-info":[{"award-number":["2025B03J0158"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,3,20]]},"abstract":"<jats:p>\n                    Therapeutic cancer vaccines show great promise for de novo induction of antigen-specific T cell responses against tumors. However, weak coordination between innate immune activation and antigen delivery remains a major obstacle to vaccine efficacy. Here, we present a cholesterol azetidine derivative\u2013assisted polymeric carrier, Aze-Chol NP, which stimulates innate immunity and primes tumor-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell responses. Mechanistically, Aze-Chol NP induces maturation and activation of dendritic cells (DCs) through the Toll-like receptor 9 (TLR9) pathway while simultaneously delivering protein or peptide antigens to DCs in lymph nodes. The Aze-Chol NP\u2013based nanovaccines markedly inhibited tumor growth and prolonged survival in melanoma and human papillomavirus tumor models. Moreover, combining the nanovaccine with an anti\u2013PD-L1 antibody produced a strong synergistic effect and long-term immune memory, achieving 80% survival beyond 100 days and complete rejection of tumor cell rechallenge. Overall, our study demonstrates that this TLR9-activating carrier provides an effective and straightforward strategy for developing potent cancer vaccines.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeb2465","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T18:01:06Z","timestamp":1774029666000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":2,"title":["TLR9-activating cholesterol azetidine derivative\u2013assisted therapeutic vaccines for cancer immunotherapy"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8516-0457","authenticated-orcid":true,"given":"Junbin","family":"Chen","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou, Guangdong 511442, P. R. 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