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Adv."],"published-print":{"date-parts":[[2026,7,17]]},"abstract":"<jats:p>\n                    The generation of megakaryocytes (MKs) from human somatic cells through chemical reprogramming represents a promising strategy for developing alternative platelet sources. Building on our prior chemical reprogramming protocol for converting erythroblasts to MKs, we established a robust method that successfully generated induced MKs (iMKs) from human cord blood\u2013derived CD3\n                    <jats:sup>+<\/jats:sup>\n                    T cells, which is a more abundant source. This method used a five\u2013small molecule cocktail containing a reprogramming booster, AZD4205, to promote erasure of T cell identity and facilitate fate transition toward MKs. T cell\u2013derived iMKs exhibited characteristic MK cellular and molecular signatures, demonstrating the capacity to produce proplatelets and release functional platelets. Single-cell RNA sequencing further revealed that iMKs were heterogeneous with distinct functional profiles, including cycling, immune, and thrombopoiesis-biased MKs. Our findings highlight an optimized chemical reprogramming strategy that enables efficient conversion of T cells to MKs, providing a practical and convenient approach to generating clinically relevant MKs and platelets.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeb3177","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T18:03:21Z","timestamp":1784138601000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Efficient chemical reprogramming of human T cells into functional megakaryocytes and platelets"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6800-2761","authenticated-orcid":true,"given":"Jinhua","family":"Qin","sequence":"first","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, 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