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Adv."],"published-print":{"date-parts":[[2026,4,3]]},"abstract":"<jats:p>\n                    Sexually transmitted infections (STIs) of the cervicovaginal mucosa are among the most common global infections. Current monolayer cell culture and animal models fail to reproduce the multilevel complexity required to investigate host-microbiota-pathogen relationships simultaneously and\/or with sufficient physiological relevance. To address this limitation, we have developed a microphysiologic system (MPS) that models human cervical tissue and its microbiota and is susceptible to infection by two prominent genital pathogens,\n                    <jats:italic toggle=\"yes\">Chlamydia trachomatis<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Neisseria gonorrhoeae<\/jats:italic>\n                    . Notably, this MPS platform recapitulates essential dynamic, polymicrobial, immune, and pathogenic features of chlamydial and gonococcal infections as they occur in humans. The low-cost MPS device requires no specialized equipment or specific expertise and was experimentally validated across multiple nonengineering, remotely located laboratories, demonstrating its transferability and reproducibility. The platform provides a tool for research into genital infections in a system that closely mimics the cervical epithelium, an important advance over existing models.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeb4864","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T17:58:57Z","timestamp":1775239137000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":5,"title":["A microphysiologic human cervical model recapitulates microbial, immune, and pathogenic properties of sexually transmitted infections"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1070-1551","authenticated-orcid":true,"given":"Katherine M.","family":"Nelson","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, University of Delaware, Newark, DE, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3065-055X","authenticated-orcid":true,"given":"Daniel J.","family":"Minahan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Delaware, Newark, DE, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vonetta L.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Center for Advanced Microbiome Research and Innovation, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA."},{"name":"Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3738-3430","authenticated-orcid":true,"given":"Ian J.","family":"Glomski","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5982-7641","authenticated-orcid":true,"given":"David J.","family":"Delgado Diaz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keena","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4503-8734","authenticated-orcid":true,"given":"Forrest C.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7340-5741","authenticated-orcid":true,"given":"Patrik M.","family":"Bavoil","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland, Baltimore, MD, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Derr\u00e9","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7738-3757","authenticated-orcid":true,"given":"Alison K.","family":"Criss","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0851-2233","authenticated-orcid":true,"given":"Jacques","family":"Ravel","sequence":"additional","affiliation":[{"name":"Center for Advanced Microbiome Research and Innovation, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA."},{"name":"Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1283-2966","authenticated-orcid":true,"given":"Jason P.","family":"Gleghorn","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Delaware, Newark, DE, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","unstructured":"CDC Sexually Transmitted Infections (STIs) (2024) [cited 24 February 2025]. 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