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Adv."],"published-print":{"date-parts":[[2026,2,20]]},"abstract":"<jats:p>\n                    Asexual reproduction of malaria parasites requires the basal complex, the equivalent of a eukaryotic contractile ring. Despite its central role, basal complex biogenesis remains largely unknown. Here, we use expansion microscopy and DNA points accumulation for imaging in nanoscale topography to investigate three transmembrane basal complex proteins in\n                    <jats:italic toggle=\"yes\">Plasmodium falciparum<\/jats:italic>\n                    \u2014basal complex transmembrane protein 1 (BTP1), BTP2, and basolateral expansion boundary (BLEB). Parasites lacking BTP2 are still enveloped by membranes but fail to separate from each other, resulting in multiorganellar mutants. We isolate the defect to a specific step during basal complex development and demonstrate that the contractile ability remains intact. By revisiting BLEB, we identify a distinct plasma membrane region that is excluded from daughter cells and associated with the basal complex. Integrating these findings, we propose a three-step model for basal complex biogenesis that highlights the specific role of BTP2 and suggests a role for the BLEB-associated membrane. This study offers a mechanistic framework for how multiple daughter cells are formed simultaneously and highlights the importance of transmembrane proteins for cell division.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeb5163","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T18:59:51Z","timestamp":1771441191000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Transmembrane proteins mediate basal complex assembly and individual daughter cell formation in malaria parasites"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4653-8602","authenticated-orcid":true,"given":"Peter S.","family":"Back","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Boston Children\u2019s Hospital, Boston, MA 02115, USA."},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9009-2298","authenticated-orcid":true,"given":"Erinn","family":"Wagner","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children\u2019s Hospital, Boston, MA 02115, USA."},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5983-2296","authenticated-orcid":true,"given":"Paula Montero","family":"Llopis","sequence":"additional","affiliation":[{"name":"MicRoN Core, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2407-5766","authenticated-orcid":true,"given":"Ramon G.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, State University of Campinas, Campinas 13083-862, Brazil."},{"name":"Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5725-0439","authenticated-orcid":true,"given":"Marco","family":"Mottinelli","sequence":"additional","affiliation":[{"name":"Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA."},{"name":"Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8992-0919","authenticated-orcid":true,"given":"Lori","family":"Ferrins","sequence":"additional","affiliation":[{"name":"Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA."},{"name":"Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5883-7271","authenticated-orcid":true,"given":"Jeffrey D.","family":"Dvorin","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children\u2019s Hospital, Boston, MA 02115, USA."},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3184"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.09.007"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.07.055"},{"key":"e_1_3_2_5_2","unstructured":"World Health Organization \u201cWorld malaria report 2024: Addressing inequity in the global malaria response\u201d (2024)."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00269"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.1.21-38.2002"},{"key":"e_1_3_2_8_2","first-page":"982","article-title":"The apicomplexan inner membrane complex","volume":"18","author":"Kono M.","year":"2013","unstructured":"M. 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Saul, P. Myler, T. Elliott, C. Kidson, Purification of mature schizonts of Plasmodium falciparum on colloidal silica gradients. Bull. World Health Organ. 60, 755\u2013759 (1982).","journal-title":"Bull. World Health Organ."},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1993.tb03313.x"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00462.2010"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01156-w"},{"key":"e_1_3_2_78_2","unstructured":"K. J. Eastman J. F. Kadow N. B. Naidu K. E. Parcella K. Peese Inhibitors of human immunodeficiency virus replication US9938271B2 2015."},{"key":"e_1_3_2_79_2","unstructured":"J. Li Z. Wang S. Xu H. Sun Y. Liu Aminopyrazine compounds as HPK1 inhibitor and the use thereof WO2021032148A1 (2021)."}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aeb5163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T16:04:57Z","timestamp":1776701097000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aeb5163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,20]]},"references-count":78,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,2,20]]}},"alternative-id":["10.1126\/sciadv.aeb5163"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aeb5163","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,20]]},"assertion":[{"value":"2025-08-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaeb5163"}}