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Adv."],"published-print":{"date-parts":[[2026,4,17]]},"abstract":"<jats:p>The cortical actin cytoskeleton serves as a structural support and force-generating machinery in cells, but the actin meshwork can also act as a barrier, hindering the delivery of extracellular substances. To address the issue of the cortical actin barrier, we engineered synthetic libraries of human \u03b2-actin\u2013derived peptides to promote actin dynamics. Here, we report the discovery of six major peptides, collectively termed actinators, that are biologically active in selective modulation of actin-related cellular activities. Using retro- and lentiviral vector transduction of CAR T cells as a model, we demonstrate that an actinator markedly enhances viral vector transduction 20- to 30-fold. An individual actinator can also selectively modulate cellular functions such as regulating surface receptors, enhancing T cell adhesion, and promoting the cellular uptake of exosomes. 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