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Adv."],"published-print":{"date-parts":[[2026,2,6]]},"abstract":"<jats:p>\n                    Huge phages are widespread in the biosphere, yet their prevalence and ecology in the human gut remain poorly characterized. Here, we report Jug (jumbo gut) phages with genomes of 360 to 402 kilobase pairs that comprise ~1.1% of the reads in human gut metagenomes, and are predicted to infect\n                    <jats:italic toggle=\"yes\">Bacteroides<\/jats:italic>\n                    and\/or\n                    <jats:italic toggle=\"yes\">Phocaeicola<\/jats:italic>\n                    . Although three of the four major groups of Jug phages shared &gt;90% genome-wide sequence identity, their large terminase subunits exhibited only 38 to 57% identity, suggesting horizontal acquisition from other phages. Over 1500 genomes of Jug phages were recovered from human and animal gut metagenomes, revealing their broad distribution, with largely shared gene content suggestive of frequent cross-animal-host transmission. Jug phages displayed high gene transcription activities, including the gene for a calcium-translocating P-type ATPase not detected previously in phages. These findings broaden our understanding of huge phages and highlight Jug phages as potential major players in gut microbiome ecology.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeb6265","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T18:59:10Z","timestamp":1770404350000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":4,"title":["Animal-associated jumbo phages as widespread and active modulators of gut microbiome ecology and metabolism"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2774-1952","authenticated-orcid":true,"given":"LinXing","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Advanced Environmental Technology, the Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 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