{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:27:13Z","timestamp":1789676833755,"version":"4.0.1"},"reference-count":78,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"22","funder":[{"name":"Elicio Therapeutics"},{"name":"UMass Chan School Fund","award":["NIH\/NHLBI 5R01 HL165787"],"award-info":[{"award-number":["NIH\/NHLBI 5R01 HL165787"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,5,29]]},"abstract":"<jats:p>\n                    Adjuvants are immunoactivators capable of shaping the magnitude and quality of antigen-specific immune responses induced by subunit immunization. Presently, there is an acute need for effective adjuvants that safely induce durable and balanced humoral and cellular responses. Here, we engineered a class of Amphiphile (AMP)\u2013modified, immunostimulatory DNA adjuvants designed for targeted delivery to lymph nodes and enhanced stimulation of TANK-binding kinase 1 (TBK1)\u2013mediated danger-sensing pathways to generate strong adaptive immunity and long-term memory with potent recall potential. AMP-DNA adjuvants induced robust interferon type-I (IFN-I)\u2013driven inflammatory environments in mouse and nonhuman primate (NHP) lymph nodes, leading to significantly enhanced cytokine secretion by polyfunctional CD8\n                    <jats:sup>+<\/jats:sup>\n                    and CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells in multiple tissues, as well as strongly elevated T helper cell 1 (T\n                    <jats:sub>H<\/jats:sub>\n                    1)\u2013associated and neutralizing antibody responses, in the absence of systemic toxicity. These results demonstrate that AMP modification enables lymph node\u2013targeted DNA adjuvants to potently activate IFN-I signaling to generate substantial cellular and humoral responses crucial for vaccine efficacy.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aec1416","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T18:00:46Z","timestamp":1780077646000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Lymph node\u2013targeted DNA engages TBK1\/IFN-I\u2013driven innate immunity to induce potent T cell responses and durable memory in mice and NHPs"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5599-8953","authenticated-orcid":true,"given":"Martin P.","family":"Steinbuck","sequence":"first","affiliation":[{"name":"Elicio Therapeutics, 451 D Street, 5th Floor, Suite 501, Boston, MA 02210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0543-5495","authenticated-orcid":true,"given":"Lochana M.","family":"Seenappa","sequence":"additional","affiliation":[{"name":"Elicio Therapeutics, 451 D Street, 5th Floor, Suite 501, Boston, MA 02210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9769-4632","authenticated-orcid":true,"given":"Wei","family":"Zhan","sequence":"additional","affiliation":[{"name":"Program in Innate Immunity, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2469-1723","authenticated-orcid":true,"given":"Erica","family":"Palmer","sequence":"additional","affiliation":[{"name":"Elicio Therapeutics, 451 D Street, 5th Floor, Suite 501, Boston, MA 02210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1693-3860","authenticated-orcid":true,"given":"Mimi M.","family":"Jung","sequence":"additional","affiliation":[{"name":"Elicio Therapeutics, 451 D Street, 5th Floor, Suite 501, Boston, MA 02210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3805-9855","authenticated-orcid":true,"given":"Aniela","family":"Jakubowski","sequence":"additional","affiliation":[{"name":"Elicio Therapeutics, 451 D Street, 5th Floor, Suite 501, Boston, MA 02210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9929-7307","authenticated-orcid":true,"given":"Xavier","family":"Cabana-Puig","sequence":"additional","affiliation":[{"name":"Elicio Therapeutics, 451 D Street, 5th Floor, Suite 501, Boston, MA 02210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa K.","family":"McNeil","sequence":"additional","affiliation":[{"name":"Elicio Therapeutics, 451 D Street, 5th Floor, Suite 501, Boston, MA 02210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8600-9548","authenticated-orcid":true,"given":"Christopher M.","family":"Haqq","sequence":"additional","affiliation":[{"name":"Elicio Therapeutics, 451 D Street, 5th Floor, Suite 501, Boston, MA 02210, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3175-609X","authenticated-orcid":true,"given":"Katherine A.","family":"Fitzgerald","sequence":"additional","affiliation":[{"name":"Program in Innate Immunity, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7044-7559","authenticated-orcid":true,"given":"Peter 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