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Adv."],"published-print":{"date-parts":[[2026,6,12]]},"abstract":"<jats:p>\n                    Nucleotide second messengers are widely used to mediate the physiology and antiphage immunity in bacteria. It was revealed that 2\u2032,3\u2032-cAMP\/2\u2032,3\u2032-cGMP synthetase activity is required for immune response in plants. However, it remains unclear whether 2\u2032,3\u2032-cNMPs are involved in the interactions between bacteria and phages. Here, we report that 2\u2032,3\u2032-cNMPs, which are synthesized by RnaA and degraded by A1S_2339, A1S_0249, and A1S_2666, play critical roles in the modulation of the physiology of\n                    <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                    . 2\u2032,3\u2032-cNMPs specifically bind to the receptor protein CRP\n                    <jats:sub>Ab<\/jats:sub>\n                    in\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    and thus affect the interaction between CRP\n                    <jats:sub>Ab<\/jats:sub>\n                    and the promoters of target genes. Furthermore, our study reveals that bacteriophages elevate 2\u2032,3\u2032-cNMPs to up-regulate the transcription of the transposases and Mu-like integrases required for bacteriophage DNA integration. Together, our results show that 2\u2032,3\u2032-cNMPs are important intracellular signals that control biological functions of\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    , and bacteriophages could hijack these signals to facilitate phage DNA integration.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aec7770","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:58:47Z","timestamp":1781287127000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Bacteriophages hijack 2\u2032,3\u2032-cNMPs to enhance transposase-mediated phage DNA integration in\n                    <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9389-4398","authenticated-orcid":true,"given":"Binbin","family":"Cui","sequence":"first","affiliation":[{"name":"Pharmacy Department, Ningbo 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