{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T18:06:21Z","timestamp":1784311581987,"version":"3.55.0"},"reference-count":84,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"29","funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2141064"],"award-info":[{"award-number":["2141064"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["K25HL169816"],"award-info":[{"award-number":["K25HL169816"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA214297"],"award-info":[{"award-number":["U01CA214297"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA268933"],"award-info":[{"award-number":["U01CA268933"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R01CA255602"],"award-info":[{"award-number":["R01CA255602"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R01CA255602"],"award-info":[{"award-number":["R01CA255602"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,7,17]]},"abstract":"<jats:p>The ability to deterministically and precisely control the lateral position of focused cell streams within a microchannel enables a wide range of biomedical applications, including cell concentration, sorting, and sensing. By leveraging engineered microvortices generated by channel ridges and fluidic lift forces, we present a microfluidic method for deterministically positioning focused cell streams to desired streamlines. Unlike inertial focusing, ridge-assisted micro positioning (RAMP) is independent of particle size and flow rate, enabling polydisperse particles to be focused to the same streamline across a broad range of flow rates. The focusing position can be precisely adjusted by altering the ridge placement and geometry, allowing for micron-level lateral shifts in a controlled manner. Applying the RAMP concept, we demonstrate clog-free microfluidic cell concentrators that can enrich single cells and clusters to desired concentration factors and show the ability to focus particles in undiluted whole blood. Together, these results establish RAMP as a versatile platform for precise cell positioning in complex biological fluids.<\/jats:p>","DOI":"10.1126\/sciadv.aec9227","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:58:48Z","timestamp":1784311128000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Ridge-assisted micro positioning of cells in a channel"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8956-5440","authenticated-orcid":true,"given":"Adriana","family":"Payan-Medina","sequence":"first","affiliation":[{"name":"Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA."},{"name":"Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Krantz Family Center for Cancer Research, Massachusetts General Hospital, Mass General Brigham Cancer Institute and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Department of Surgery, Massachusetts General Hospital, Mass General Brigham and Harvard Medical School, Boston, Massachusetts 02114."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7434-787X","authenticated-orcid":true,"given":"Victor R.","family":"Putaturo","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Krantz Family Center for Cancer Research, Massachusetts General Hospital, Mass General Brigham Cancer Institute and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Department of Surgery, Massachusetts General Hospital, Mass General Brigham and Harvard Medical School, Boston, Massachusetts 02114."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6419-3361","authenticated-orcid":true,"given":"Sajad R.","family":"Bazaz","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Krantz Family Center for Cancer Research, Massachusetts General Hospital, Mass General Brigham Cancer Institute and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Department of Surgery, Massachusetts General Hospital, Mass General Brigham and Harvard Medical School, Boston, Massachusetts 02114."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9086-8908","authenticated-orcid":true,"given":"Quinn E.","family":"Cunneely","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Krantz Family Center for Cancer Research, Massachusetts General Hospital, Mass General Brigham Cancer Institute and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Department of Surgery, Massachusetts General Hospital, Mass General Brigham and Harvard Medical School, Boston, Massachusetts 02114."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9397-0537","authenticated-orcid":true,"given":"Varun","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"School of Engineering and Applied Sciences and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Toner","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Department of Surgery, Massachusetts General Hospital, Mass General Brigham and Harvard Medical School, Boston, Massachusetts 02114."},{"name":"Shriners Children\u2019s, Boston, MA 02114, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5351-1339","authenticated-orcid":true,"given":"Avanish","family":"Mishra","sequence":"additional","affiliation":[{"name":"Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Krantz Family Center for Cancer Research, Massachusetts General Hospital, Mass General Brigham Cancer Institute and Harvard Medical School, Charlestown, Massachusetts 02129, USA."},{"name":"Department of Surgery, Massachusetts General Hospital, Mass General Brigham and Harvard Medical School, Boston, Massachusetts 02114."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc41104k"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05063"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2017.08.012"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201300502"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.7.011205.135108"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpim.40"},{"key":"e_1_3_2_8_2","unstructured":"A. L. Givan \u201cFlow Cytometry: An Introduction\u201d in Flow Cytometry Protocols T. S. Hawley R. G. Hawley Eds. (Humana Press Totowa NJ 2011) vol. 699 of Methods in Molecular Biology pp. 1\u201329. https:\/\/link.springer.com\/10.1007\/978-1-61737-950-5_1."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24361"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23964"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac100387b"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1039\/B919495A"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201202413"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","unstructured":"K. C. M. Lee B. M. F. Chung D. M. D. Siu S. C. K. Ho D. K. H. Ng K. K. Tsia Dispersion-free inertial focusing (DIF) for high-yield polydisperse micro-particles filtration and analysis. bioRxiv 2024.01.20.576445 (2024). https:\/\/doi.org\/10.1101\/2024.01.20.576445.","DOI":"10.1101\/2024.01.20.576445"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.4974903"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-009-9374-9"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-121813-120704"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep03340"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1066238"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0257389"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc41248a"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.102.094503"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-28495-2"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5120"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep11018"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c03474"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep11300"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-022-01351-y"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2025.07.008"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-55140-x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-015-0018-y"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.4758131"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b02277"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-fluid-010816-060150"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1039\/C0LC00102C"},{"key":"e_1_3_2_37_2","first-page":"32","article-title":"The Deborah and Weissenberg numbers","volume":"53","author":"Poole R.","year":"2012","unstructured":"R. Poole, The Deborah and Weissenberg numbers. Rheol. Bull. 53, 32\u201339 (2012).","journal-title":"Rheol. Bull."},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3lc50679g"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1039\/B301019H"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2006388117"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00079-2"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s44222-025-00334-1"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900736"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990110203"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10404-013-1306-6"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep38809"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41378-018-0005-6"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1088\/0960-1317\/23\/8\/085023"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2019.09.008"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1039\/C9LC01122F"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep41153"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1039\/b701227f"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10404-017-1890-y"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1039\/D3SM01117H"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4LC01150C"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc21225k"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2018.03.002"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075901"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01807-z"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b05143"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10404-023-02645-4"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10404-021-02425-y"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5LC01100K"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10404-018-2174-x"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23765"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6LC00843G"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b01432"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19328"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1039\/D2LC00197G"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ces.2023.118826"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep04527"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704958104"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1039\/C3LC50617G"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1039\/b908271a"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.5125264"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1039\/b807107a"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2025.114570"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c04179"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2022.132284"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.3390\/mi12010097"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.11.081"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4LC01216J"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8LC00973B"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.135892"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b03851"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aec9227","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:59:45Z","timestamp":1784311185000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aec9227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,17]]},"references-count":84,"journal-issue":{"issue":"29","published-print":{"date-parts":[[2026,7,17]]}},"alternative-id":["10.1126\/sciadv.aec9227"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aec9227","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,17]]},"assertion":[{"value":"2025-10-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaec9227"}}