{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:28:21Z","timestamp":1791325701629,"version":"4.3.1"},"reference-count":58,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"18","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32225047"],"award-info":[{"award-number":["32225047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20510"],"award-info":[{"award-number":["U22A20510"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32521006"],"award-info":[{"award-number":["32521006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402804"],"award-info":[{"award-number":["32402804"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Double First-class Discipline Promotion Project","award":["2023B10564001"],"award-info":[{"award-number":["2023B10564001"]}]},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture","award":["NT2025004"],"award-info":[{"award-number":["NT2025004"]}]},{"name":"Yuelushan Laboratory Talent Program","award":["2025RC3002"],"award-info":[{"award-number":["2025RC3002"]}]},{"name":"Independent Research Fund of State Key Laboratory of Swine and Poultry Breeding Industry","award":["2025ZQQZ-G26"],"award-info":[{"award-number":["2025ZQQZ-G26"]}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["2025RC4006"],"award-info":[{"award-number":["2025RC4006"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,5,1]]},"abstract":"<jats:p>\n                    <jats:sc>d<\/jats:sc>\n                    -amino acids have been detected in various tissues; however, whether\n                    <jats:sc>d<\/jats:sc>\n                    -amino acids shape immune cell (e.g., macrophages) function remains undefined. Here, we demonstrated that inflammatory macrophages decrease mRNA expression of\n                    <jats:sc>d<\/jats:sc>\n                    -amino acid oxidase (DAAO) and\n                    <jats:sc>d<\/jats:sc>\n                    -aspartate oxidase (DDO) through nuclear factor \u03baB (NF-\u03baB) signaling. Notably, inhibition of DAAO or DDO increases the concentration of intracellular\n                    <jats:sc>d<\/jats:sc>\n                    -amino acids, consequently suppressing IL-1\u03b2 release. Mechanistically,\n                    <jats:sc>d<\/jats:sc>\n                    -amino acids inhibit the formation of gasdermin D (GSDMD) oligomer via GSDMD-K146 acetylation.\n                    <jats:sc>d<\/jats:sc>\n                    -amino acids directly bind and increase the enzyme activity of mitochondrial pyruvate dehydrogenase (PDH), resulting in acetyl\u2013coenzyme A production for acetylation. Consistently,\n                    <jats:sc>d<\/jats:sc>\n                    -Ala\/\n                    <jats:sc>d<\/jats:sc>\n                    -Glu supplementation or myeloid-specific deletion of DDO attenuates lipopolysaccharides (LPS)\u2013induced sepsis in mice. Collectively, our study reveals a mechanism involving acetylation mediated by\n                    <jats:sc>d<\/jats:sc>\n                    -amino acids in regulation of macrophage function, providing a potential therapeutic strategy for treating macrophage-associated inflammatory diseases.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aed1676","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T17:58:27Z","timestamp":1777485507000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":2,"title":["<scp>d<\/scp>\n                    -amino acids restrain macrophage IL-1\u03b2 release through gasdermin D acetylation"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6820-2497","authenticated-orcid":true,"given":"Zebiao","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory of Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qilin","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory of Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhao","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory of Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2765-1175","authenticated-orcid":true,"given":"Jian","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory of Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingnan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory of Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meimei","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory of Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ifen","family":"Hung","sequence":"additional","affiliation":[{"name":"Anyou Biotechnology Group Co. Ltd., Taicang, China."},{"name":"Centree Bio-tech (Wuhan) Co. Ltd., Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxue","family":"Liu","sequence":"additional","affiliation":[{"name":"Anyou Biotechnology Group Co. Ltd., Taicang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8145-8907","authenticated-orcid":true,"given":"Wenkai","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory of Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China."},{"name":"Yuelushan Laboratory, Changsha 410128, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0124-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109720"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-022-00862-5"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-081022-021207"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03478-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0128-5"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-081619-104850"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.125"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111770"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.753092"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe9274"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-022-2296-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abd6480"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2023.09.001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2300817120"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01757-9"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.123.063760"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30228"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-021-00145-1"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2015.04.028"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-021-00780-y"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abj3859"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aat2738"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb9818"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0081-3"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16348-9"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112008"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.09.013"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature18629"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-020-01681-0"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10352-4"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2210929120"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13668-3"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.06.019"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2018.03.014"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.09.034"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01302-6"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.833080"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-023-04971-w"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2215921119"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2024.01.002"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.06.028"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-023-01656-1"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.06.038"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2017.04.004"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1503-x"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2019.05.007"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-025-2999-1"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.01.009"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adn2867"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.06.023"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.01.014"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201901703R"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01803-x"},{"key":"e_1_3_2_56_2","first-page":"e2084131176","article-title":"Defining and targeting tumor-associated macrophages in malignant mesothelioma","volume":"120","author":"Wu L.","year":"2023","unstructured":"L. Wu, M. Kohno, J. Murakami, A. Zia, J. Allen, H. Yun, M. Chan, C. Baciu, M. Liu, V. Serre-Beinier, M. De Palma, E. Felley-Bosco, J. Yeung, T. J. Pugh, M. de Perrot, Defining and targeting tumor-associated macrophages in malignant mesothelioma. Proc. Natl. Acad. Sci. U.S.A. 120, e2084131176 (2023).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2024.03.055"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.241"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-025-02236-9"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aed1676","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T17:59:50Z","timestamp":1777485590000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aed1676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,1]]},"references-count":58,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2026,5,1]]}},"alternative-id":["10.1126\/sciadv.aed1676"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aed1676","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,1]]},"assertion":[{"value":"2025-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaed1676"}}