{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:21:34Z","timestamp":1787768494648,"version":"build-2784847793"},"reference-count":58,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"35","funder":[{"DOI":"10.13039\/100018703","name":"European Innovation Council","doi-asserted-by":"publisher","award":["OPTIPATH 7D, 101185769"],"award-info":[{"award-number":["OPTIPATH 7D, 101185769"]}],"id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006095","name":"Helse S\u00f8r-\u00d8st RHF","doi-asserted-by":"publisher","award":["2022046"],"award-info":[{"award-number":["2022046"]}],"id":[{"id":"10.13039\/501100006095","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Norges Forskningsr\u00e5det","award":["327571, 295910"],"award-info":[{"award-number":["327571, 295910"]}]},{"name":"Nationalforeningen for folkehelse","award":["Demenspris 2025, Norway"],"award-info":[{"award-number":["Demenspris 2025, Norway"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,8,28]]},"abstract":"<jats:p>\n                    Mass spectrometry imaging (MSI) produces high-dimensional molecular data, but practical interpretation remains limited by visualizations that incompletely preserve global structure. We present MSI-VISUAL, an open-source framework for interactive MSI analysis that integrates truthful dimensionality reduction visualizations with region-of-interest selection, statistical comparison, and direct mass\/charge ratio (\n                    <jats:italic toggle=\"yes\">m\/z<\/jats:italic>\n                    ) mapping. MSI-VISUAL introduces four visualization strategies: SALO and SPEAR (optimization-based methods designed to improve global structure preservation across distance metrics) and TOP3 and PR3D (lightweight approaches for memory-efficient, rapid visualization of large datasets). Across benchmarks and lipidomics case studies, including mouse brain and kidney pathology examples, the proposed methods outperform commonly used alternatives in our benchmarks, improve detection of subtle tissue differences, and reveal fine molecular-anatomical patterns that support biological insights. These results establish MSI-VISUAL as a scalable framework for discovery-oriented and diagnostic MSI workflows.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aed3650","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:59:39Z","timestamp":1787767179000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Truthful visualizations for mass spectrometry imaging enable high-spatial-resolution interactive\n                    <i>m\/z<\/i>\n                    mapping and exploration"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1291-2520","authenticated-orcid":true,"given":"Jacob","family":"Gildenblat","sequence":"first","affiliation":[{"name":"Pahnke Lab, Oslo, Norway."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7355-4213","authenticated-orcid":true,"given":"Jens","family":"Pahnke","sequence":"additional","affiliation":[{"name":"Pahnke Lab, Oslo, Norway."},{"name":"Translational Neurodegeneration Research and Neuropathology Lab, Department of Clinical Medicine, Medical Faculty, University of Oslo, Sognsvannsveien 20, Oslo NO-0372, Norway."},{"name":"Section of Neuropathology Research, Department of Pathology, Division of Laboratory Medicine, Oslo University Hospital, Sognsvannsveien 20, Oslo NO-0372, Norway."},{"name":"Institute of Nutritional Medicine, University of L\u00fcbeck and University Medical Center Schleswig-Holstein, Ratzeburger Allee 160, L\u00fcbeck D-23538, Germany."},{"name":"Department of Neuromedicine and Neuroscience, The Faculty of Medicine and Life Sciences, University of Latvia, Jelgavas iela 3, R\u012bga LV-1004, Latvia."},{"name":"Department of Neurobiology, School of Neurobiology, Biochemistry and Biophysics, The Georg S. Wise Faculty of Life Sciences, Tel Aviv University, G. S. Wise Street, Ramat Aviv IL-6997801, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.5702\/massspectrometry.A0102"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biodatasci-011420-031537"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02392-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s44303-024-00025-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adu3730"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.07.026"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-02070-z"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2023.117105"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","unstructured":"T. Alexandrov K. Ovchinnikova A. Palmer V. Kovalev A. Tarasov L. Stuart R. Nigmetzianov D. Fay Key METASPACE contributors M. Gaudin C. G. Lopez M. Vetter J. Swales M. Bokhart M. Kompauer J. M. Kenzie L. Rappez D. Velickovic R. Lavigne G. Zhang D. Thinagaran E. Ruhland M. Sans S. Triana D. A. Sammour S. Aboulmagd C. Bagger N. Strittmatter A. Rigopoulos E. Gemperline A. M. Joensen B. Geier C. Quiason E. Weaver M. Prasad B. Balluff K. Nagornov L. Li M. Linscheid C. Hopf D. Heintz M. Liebeke B. Spengler B. Boughton C. Janfelt K. Sharma C. Pineau C. Anderton S. Ellis M. Becker J. P\u00e1ncz\u00e9l G. D. Violante D. Muddiman R. Goodwin L. Eberlin Z. Takats S. Shahidi-Latham METASPACE: A community-populated knowledge base of spatial metabolomes in health and disease. bioRxiv 539478 [Preprint] (2019). https:\/\/doi.org\/10.1101\/539478.","DOI":"10.1101\/539478"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","unstructured":"O. Bachem M. Lucic A. 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