{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:24:16Z","timestamp":1790666656364,"version":"4.1.0"},"reference-count":49,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"22","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AG076075"],"award-info":[{"award-number":["R01AG076075"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5T32DK091317"],"award-info":[{"award-number":["5T32DK091317"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5T32DK091317"],"award-info":[{"award-number":["5T32DK091317"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["25PRE1362056"],"award-info":[{"award-number":["25PRE1362056"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["26POST1548675"],"award-info":[{"award-number":["26POST1548675"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,5,29]]},"abstract":"<jats:p>\n                    Aged skeletal muscle has a diminished capacity to recover after disuse. Although muscle regrowth requires coordinated interactions between immune and progenitor cells, the mechanisms of impaired remodeling in aged skeletal muscle remain poorly understood yet possibly involve the accumulation of senescent cells. We used a flow cytometry approach coupled with scRNAseq to determine the muscle senescent cell identity and transcriptional landscape during skeletal muscle recovery following disuse atrophy. Young and aged mice underwent 14 days of hindlimb unloading followed by reloading (7 or 14 days). At recovery, old mice showed smaller myofibers and abnormal muscle macrophage dynamics corresponding to greater collagen content. These outcomes coincided with elevated markers of muscle senescence (p21 and \u03b3H2AX) and increased SPiDER-\u03b2-Gal\n                    <jats:sup>+<\/jats:sup>\n                    cells, which inversely correlated with muscle mass. Single-cell resolution of SPiDER\n                    <jats:sup>+<\/jats:sup>\n                    cells unmasked several senescent interstitial muscle vascular and stromal populations. Senescent interstitial cell populations were enriched in aged muscle and displayed a senescence-associated secretory phenotype (SASP) across multiple stromal, vascular, and immune cell types. Senolytic treatment reduced overall senescent cell burden, attenuated macrophage accumulation, and restored muscle mass and function in aged mice following disuse. These findings identify a multicellular senescence environment within the muscle interstitial niche as a hallmark of impaired muscle recovery following disuse.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aed5255","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T18:02:22Z","timestamp":1779904942000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":3,"title":["Multicellular senescence impairs skeletal muscle recovery following disuse in aging"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1047-5897","authenticated-orcid":true,"given":"Paul-Emile","family":"Bourrant","sequence":"first","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, University of Utah, 250 South 1850 East, Salt Lake City, UT 84112, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5339-8051","authenticated-orcid":true,"given":"Elena M.","family":"Yee","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, University of Utah, 250 South 1850 East, Salt Lake City, UT 84112, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9482-7815","authenticated-orcid":true,"given":"Zachary J.","family":"Fennel","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy and Athletic Training, University of Utah, 520 Wakara Way, Salt Lake City, UT 84108, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4025-0077","authenticated-orcid":true,"given":"Robert J.","family":"Castro","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, University of Utah, 250 South 1850 East, Salt Lake City, UT 84112, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8618-5092","authenticated-orcid":true,"given":"Chad M.","family":"Skiles","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy and Athletic Training, University of Utah, 520 Wakara Way, Salt Lake City, UT 84108, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1298-4257","authenticated-orcid":true,"given":"Jonathan J.","family":"Petrocelli","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy and Athletic Training, University of Utah, 520 Wakara Way, Salt Lake City, UT 84108, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6732-0217","authenticated-orcid":true,"given":"Naomi M. 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P.","family":"de Hart","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, University of Utah, 250 South 1850 East, Salt Lake City, UT 84112, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A.","family":"Lesniewski","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Geriatrics, University of Utah, 30 North Mario Capecchi Dr, 2nd Floor North, Salt Lake City, Utah 84112, USA."},{"name":"Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, 95 South 2000 East, Bldg. 500, Salt Lake City, UT 84112-5000, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8580-8660","authenticated-orcid":true,"given":"Anna E.","family":"Beaudin","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Internal Medicine and Program in Molecular Medicine, University of Utah, Salt Lake City, UT 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