{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:26:28Z","timestamp":1780511188793,"version":"3.54.1"},"reference-count":98,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"23","funder":[{"DOI":"10.13039\/501100010730","name":"Temasek Life Sciences Laboratory","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010730","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,6,5]]},"abstract":"<jats:p>\n                    Sex-determination mechanisms and their master regulators are diverse among vertebrates. Components of downstream regulatory pathways can sometimes be co-opted as new upstream triggers. Here, we report a notable finding of cross-pathway recruitment: A gene from the female differentiation cascade was repurposed as a male master sex-determining (MSD) gene. We identify\n                    <jats:italic toggle=\"yes\">figlaY<\/jats:italic>\n                    , a Y-linked truncated duplicate of the female-associated\n                    <jats:italic toggle=\"yes\">figla<\/jats:italic>\n                    , as the MSD gene in Mozambique tilapia.\n                    <jats:italic toggle=\"yes\">figlaY<\/jats:italic>\n                    originated through duplication and translocation in an ancestral cichlid lineage and subsequently introgressed into multiple lineages. Transposable elements facilitated\n                    <jats:italic toggle=\"yes\">figlaY<\/jats:italic>\n                    \u2019s emergence by mediating duplication, truncation, and regulatory rewiring. Functionally, FIGLAY suppresses the\n                    <jats:italic toggle=\"yes\">zp2<\/jats:italic>\n                    promoter activated by the FIGLA\/E12 heterodimer, acting as a dominant-negative regulator that inhibits ovarian differentiation and promotes testis development. This discovery reveals an unprecedented evolutionary route from female to male sex determination, i.e., a \u201cgenetic defection\u201d from an initially female pathway. This previously unknown mechanism expands the vertebrate sex-determination toolkit.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aed6242","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:59:03Z","timestamp":1780509543000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Transposons drove the evolution of a male sex-determining gene from a female gene with deep introgression across cichlids"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3363-5753","authenticated-orcid":true,"given":"Le","family":"Wang","sequence":"first","affiliation":[{"name":"Temasek Life Sciences Laboratory, Singapore 117604, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9998-4331","authenticated-orcid":true,"given":"Fei","family":"Sun","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, Singapore 117604, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2206-0445","authenticated-orcid":true,"given":"Zituo","family":"Yang","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, Singapore 117604, Singapore."},{"name":"College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9188-3627","authenticated-orcid":true,"given":"May","family":"Lee","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, Singapore 117604, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7858-3267","authenticated-orcid":true,"given":"Joey","family":"Wong","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, Singapore 117604, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4717-377X","authenticated-orcid":true,"given":"Shadame","family":"Yeo","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, Singapore 117604, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7852-4133","authenticated-orcid":true,"given":"Yanfei","family":"Wen","sequence":"additional","affiliation":[{"name":"Temasek Life Sciences Laboratory, Singapore 117604, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0888-8193","authenticated-orcid":true,"given":"Axel","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Konstanz, 78457 Konstanz, Germany."},{"name":"Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, USA."},{"name":"CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China."},{"name":"MOE Key Laboratory of Freshwater Fish Reproduction and Development, College of Fisheries, School of Life Sciences, Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Chongqing 400715, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0903-4736","authenticated-orcid":true,"given":"Francesc","family":"Piferrer","sequence":"additional","affiliation":[{"name":"Institute of Marine Sciences (ICM), Spanish National Research Council (CSIC), 08003 Barcelona, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9882-5948","authenticated-orcid":true,"given":"Manfred","family":"Schartl","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University of Wuerzburg, 97074 Wuerzburg, 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