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Adv."],"published-print":{"date-parts":[[2026,5,22]]},"abstract":"<jats:p>High-fidelity profiling of intracellular biomarkers is crucial for deciphering tumorigenesis and progression, as well as classifying cell types. Conventional techniques suffer from high background interference and poor stability due to the scattered, point-like signals. Herein, we present a DNA tile\u2013driven in vivo logical encoding self-assembly (TILES) strategy for robust and signal-amplified imaging of multiple biomarkers in cancer cells and tumor-bearing mice. Capitalizing on AND-gated logic operations, the TILES assay enables confined recognition of dual biomarkers to initiate the intracellular self-assembly of DNA scaffolds, yielding nanotube-like signals. Owing to the compact nucleic acid framework, DNA scaffolds exhibit an over sevenfold nuclease resistance enhancement than double-stranded DNA probes. 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