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Adv."],"published-print":{"date-parts":[[2026,10,2]]},"abstract":"<jats:p>Intraoperative fluorescence imaging holds great promise for achieving complete cytoreduction in ovarian cancer, but its clinical utility is currently constrained by the limitations of existing imaging agents. In particular, the leading clinical probe, OTL38, operates in the near-infrared I (NIR-I; 700 to 1000\u00a0nanometers) window, which suffers from limited tissue penetration and suboptimal resolution. Here, a folate receptor \u03b1 (FR\u03b1)\u2013targeted NIR-II (1000 to 3000\u00a0nanometers) imaging probe was developed by incorporating a 4-(4-iodophenyl)butanoic acid moiety. This structural modification enables albumin binding, thereby prolonging systemic circulation and enhancing accumulation in FR\u03b1-positive tumors. In murine models of peritoneal metastasis, our probe enabled precise visualization of occult lesions undetectable by the naked eye. Compared to OTL38, this probe demonstrated superior tumor detection sensitivity (90.20% versus 78.75%) and allowed for the reliable identification of elusive lesions less than or equal to 3\u00a0millimeters in diameter. These findings highlight the potential of albumin-binding NIR-II imaging strategies to overcome the limitations of current clinical probes and enhance precision fluorescence\u2013guided surgery.<\/jats:p>","DOI":"10.1126\/sciadv.aed7722","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:59:16Z","timestamp":1790963956000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["A folate receptor\u2013targeted NIR-II probe improves intraoperative imaging and surgery of metastatic ovarian cancer"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8747-4783","authenticated-orcid":true,"given":"Wanying","family":"Li","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, P. R. 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China."},{"name":"Department of Diagnostic Radiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117544, Singapore."},{"name":"Theranostics Centre of Excellence, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 138667, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3747-6518","authenticated-orcid":true,"given":"Yuhuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, P. R. China."},{"name":"Laboratory of Female Reproductive Health, The First Hospital of Jilin University, Changchun 130021, P. R. 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