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Adv."],"published-print":{"date-parts":[[2026,6,19]]},"abstract":"<jats:p>\n                    Catheter-associated urinary tract infections (CAUTIs) account for approximately 80% of urinary tract infections (UTI) and can lead to adverse outcomes. Most CAUTIs are polymicrobial with resilient communities maintaining a consistent composition of species over time. However, the mechanisms promoting persistence are poorly understood. Here, we examine how a chemical interaction between\n                    <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    can explain their high rate of co-occurrence on long-term indwelling catheters. Sequence analyses of longitudinal isolates from several patients coinfected with\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    revealed that despite frequent replacement, catheters became recolonized with the same or a nearly identical consortium of strains throughout the collection period. Using artificial urine medium (AUM), monoculture revealed that the\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    isolates grew robustly and formed biofilm, while the\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    isolates grew poorly and did not form biofilm. However, coculture of paired isolates resulted in enhanced\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    growth and biofilm, which could be reproduced by supplementing\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    with\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    conditioned AUM supernatant (KpAUMSup). Analyses using comparative transcriptomics, mutant strains, and chemical inhibitors with cell culture and murine CAUTI models revealed that (i) KpAUMSup, but not AUM, stimulated expression of the\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    Fsr quorum sensing system; (ii) Fsr was required for\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    to respond to KpAUMSup; (iii)\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    cultured in KpAUMSup was more efficient in initiating CAUTI; and (iv) disruption of Fsr inhibited initiation of CAUTI. This interspecies signaling may help explain the high rate of co-colonization of these CAUTI pathogens and highlights therapeutic strategies to treat polymicrobial CAUTI.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aee0675","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T18:00:33Z","timestamp":1781892033000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":4,"title":["Metabolic cross-talk promotes persistence of\n                    <i>Enterococcus<\/i>\n                    in a model of polymicrobial catheter-associated urinary tract infection"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4770-2144","authenticated-orcid":true,"given":"Zongsen","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7389-7440","authenticated-orcid":true,"given":"Jerome S.","family":"Pinkner","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe L. P.","family":"Obernuefemann","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3501-0063","authenticated-orcid":true,"given":"Kent R.","family":"Kleinschmidt","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6903-0273","authenticated-orcid":true,"given":"Denise A.","family":"Sanick","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne M.","family":"Hickerson","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen W.","family":"Dodson","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1755-3202","authenticated-orcid":true,"given":"Jeffrey P.","family":"Henderson","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Infectious Diseases, Center for Women\u2019s Infectious Diseases Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8785-564X","authenticated-orcid":true,"given":"Scott J.","family":"Hultgren","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1171-0772","authenticated-orcid":true,"given":"Michael G.","family":"Caparon","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.02.010"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2023.02.009"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.7044"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1641\/0006-3568(2001)051[0235:MFATSO]2.0.CO;2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-019-0089-2"},{"key":"e_1_3_2_7_2","first-page":"20240612","article-title":"Trophic interactions in microbiomes influence plant host population size and ecosystem function","volume":"291","author":"Tan J.","year":"2024","unstructured":"J. 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