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Adv."],"published-print":{"date-parts":[[2026,10,9]]},"abstract":"<jats:p>Tempo-spatially controlled culture and assembly of cellular spheroids are fundamental in engineering tissue-like assembloids for scalable application, yet remain a technical challenge. Here, we show self-buoyant culture of cell spheroids and programmable assembly of multi-spheroid by microbubbles in a high-throughput, external field-free and miniaturized format. This approach allows one-step engineering of biocompatible cell-adhesive buoyancy interface that lifts the self-adaptive levitation growth of reliable one-drop-one-spheroid in an array without requiring any external fields. Using this self-floating spheroidal microarray, we find that a facile droplet \u2018kiss\u2019 facilitates the buoyancy-driven ultrafast transfer (\u223c1 s) of spheroids between top-down adjacent droplets, thus achieving rapid spheroid relocation, media exchange, and drug administration in parallel. Such a self-buoyant approach allows programmable horizontal\/vertical bioassembly and enhanced fusion of homo\/heterogeneous multi-spheroids into different \u201cbuoyantoid\u201d patterns via sequential flash transfer along droplet array. This self-powered design provides a promising tool to enable mass production and smart manipulation of encoded assembloids for precision medicine, tissue engineering and high-throughput drug screening.<\/jats:p>","DOI":"10.1126\/sciadv.aee3306","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:58:33Z","timestamp":1791395913000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Self-buoyant cell-spheroid culture programming multi-spheroid assembly"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0539-3507","authenticated-orcid":true,"given":"Yuanhang","family":"Xiang","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Pharmaceutical Precision Detection and Screening, School of Pharmacy, State Key Laboratory of Targeting Oncology, Guangxi Medical University, Nanning 530021, China."},{"name":"School of Chemistry and Chemical 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