{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:34:30Z","timestamp":1784676870953,"version":"3.55.0"},"reference-count":43,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"19","funder":[{"DOI":"10.13039\/100007493","name":"University of Colorado Boulder","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007493","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Colorado Boulder Libraries Open Access Fund"},{"name":"Schmidt Sciences, LLC"},{"name":"Colorado Shared Instrumentation in Nanofabrication and Characterization"},{"name":"Biofrontiers Advanced Light Microscopy Core"}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,5,8]]},"abstract":"<jats:p>\n                    Bioluminescence offers a powerful tool for real-time, label-free sensing for living materials. However, conventional approaches often rely on mechanical stimulation, which is difficult to standardize, localize, and sustain. Here, we introduce a chemical strategy to stimulate and sustain bioluminescence in the marine dinoflagellate\n                    <jats:italic toggle=\"yes\">Pyrocystis lunula<\/jats:italic>\n                    , enabling durable, adaptive, light-emitting living materials. By embedding\n                    <jats:italic toggle=\"yes\">P. lunula<\/jats:italic>\n                    into 3D-printed, alginate scaffolds, we engineered architecturally stable constructs with long-term cellular retention, viability, and light-emitting capacity. Exposure to acidic and basic environments produced chemically encoded bioluminescent signatures: Acid triggered localized, persistent emission, while base induced diffuse, biphasic emission indicative of cellular stress. Notably, combining chemical and mechanical stimulation led to enhanced total bioluminescence emission, achieving greater amplitude and duration of light emission without compromising cell mechanoresponsiveness. Longitudinal studies over 4 weeks demonstrated that our materials retain responsiveness and structural integrity across repeated stimulation cycles, overcoming single-use limitations. Together, these findings establish a chemistry-controlled platform for light-emitting living materials for biosensing, soft robotics, and environmental monitoring.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aee3907","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T17:59:26Z","timestamp":1778090366000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Chemical stimulation sustains bioluminescence of living light materials"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0785-2332","authenticated-orcid":true,"given":"Giulia","family":"Brachi","sequence":"first","affiliation":[{"name":"Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, 1111 Engineering Drive, Boulder, CO 80309, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8929-9718","authenticated-orcid":true,"given":"Jessica","family":"McKean","sequence":"additional","affiliation":[{"name":"Materials Science and Engineering Program, University of Colorado Boulder, 4001 Discovery Drive, Boulder, CO 80303, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4924-8467","authenticated-orcid":true,"given":"Cheng Pau","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, 1111 Engineering Drive, Boulder, CO 80309, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4987-2558","authenticated-orcid":true,"given":"Joy","family":"Edwin-Ezeh","sequence":"additional","affiliation":[{"name":"Materials Science and Engineering Program, University of Colorado Boulder, 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