{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:26:30Z","timestamp":1792787190061,"version":"4.5.0"},"reference-count":86,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"39","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82502776"],"award-info":[{"award-number":["82502776"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372296"],"award-info":[{"award-number":["82372296"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2021-I2M-1-037"],"award-info":[{"award-number":["2021-I2M-1-037"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2023-I2M-2-001"],"award-info":[{"award-number":["2023-I2M-2-001"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2024-I2M-TS-020"],"award-info":[{"award-number":["2024-I2M-TS-020"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2023-PT310-04"],"award-info":[{"award-number":["2023-PT310-04"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2023-PT180-01"],"award-info":[{"award-number":["2023-PT180-01"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2025-I2M-XHJC-033"],"award-info":[{"award-number":["2025-I2M-XHJC-033"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National key R&D program of China","award":["2023YFE0113400"],"award-info":[{"award-number":["2023YFE0113400"]}]},{"name":"Scientific Research Innovation Capability Support Project for Young Faculty","award":["ZYGXQNJSKYCXNLZCXM-H3"],"award-info":[{"award-number":["ZYGXQNJSKYCXNLZCXM-H3"]}]},{"name":"Beijing Natural Science Foundation","award":["L248066"],"award-info":[{"award-number":["L248066"]}]},{"name":"Beijing Natural Science Foundation","award":["5244051"],"award-info":[{"award-number":["5244051"]}]},{"name":"Postdoctoral Fellowship Program of China Postdoctoral Science Foundation","award":["GZB20240073"],"award-info":[{"award-number":["GZB20240073"]}],"id":[{"id":"https:\/\/ror.org\/0426zh255","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["2025ZD01900700"],"award-info":[{"award-number":["2025ZD01900700"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,9,25]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">tuberculosis<\/jats:italic>\n                    , Mtb) is an intracellular bacterium persisting in macrophages, which can cause chronic infection and drug resistance. To address transmembrane limitation and improve treatment accuracy, we employed the\n                    <jats:italic toggle=\"yes\">Photorhabdus<\/jats:italic>\n                    virulence cassette (PVC), a typical bacterial extracellular Contractile Injection System (eCIS), to deliver antimicrobial peptides (AMPs) into macrophages for intracellular mycobacteria clearance. We demonstrated that the survival of various intracellular mycobacteria, including\n                    <jats:italic toggle=\"yes\">Mycobacterium smegmatis<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">M. smegmatis<\/jats:italic>\n                    ),\n                    <jats:italic toggle=\"yes\">Mycobacterium bovis<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                    ),\n                    <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                    and its isoniazid-resistant strain, can be significantly inhibited by PVC-mediated AMP transmembrane delivery. The engineered PVC, loaded with mycobacteriophage-derived AMPs and targeting alveolar macrophages (AMs) through binding to surface receptor CD11b, eliminated AM-dwelling mycobacteria and alleviated inflammation in murine infection models. Notably, the pulmonary mycobacterial load in\n                    <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                    H37Rv-infected mice was reduced by more than tenfold. In summary, this work represents a pioneering application of engineered PVC-based intracellular delivery of biomacromolecules into macrophages, providing a novel therapeutic strategy against mycobacterial infection.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aee8463","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:59:49Z","timestamp":1790186389000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Transmembrane delivery of antimicrobial peptides by engineered eCIS into macrophages for intracellular mycobacteria elimination"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1874-3654","authenticated-orcid":true,"given":"Qiqi","family":"Wu","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1609-6605","authenticated-orcid":true,"given":"Ruiwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7310-2751","authenticated-orcid":true,"given":"Mei-Yi","family":"Yan","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8518-0829","authenticated-orcid":true,"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Health and Multimorbidity, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Feng","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Yang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Yuan","family":"Ding","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5790-7071","authenticated-orcid":true,"given":"Yi-Cheng","family":"Sun","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4486-9424","authenticated-orcid":true,"given":"Lingjun","family":"Zhan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Health and Multimorbidity, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3586-6344","authenticated-orcid":true,"given":"Qi","family":"Jin","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5161-6153","authenticated-orcid":true,"given":"Feng","family":"Jiang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (MOE), National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","unstructured":"World Health Organization (WHO) \u201cGlobal tuberculosis report 2025\u201d. https:\/\/www.who.int\/teams\/global-programme-on-tuberculosis-and-lung-health\/tb-reports\/global-tuberculosis-report-2025."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24065202"},{"key":"e_1_3_2_4_2","first-page":"729","article-title":"The diagnosis and treatment of tuberculosis","volume":"116","author":"Su\u00e1rez I.","year":"2019","unstructured":"I. Su\u00e1rez, S. M. F\u00fcnger, S. Kr\u00f6ger, J. Rademacher, G. F\u00e4tkenheuer, J. Rybniker, The diagnosis and treatment of tuberculosis. Dtsch. Arztebl. Int. 116, 729\u2013735 (2019).","journal-title":"Dtsch. Arztebl. Int."},{"key":"e_1_3_2_5_2","first-page":"245","article-title":"Update on the treatment of tuberculosis","volume":"163","author":"Mart\u00ednez-Campreci\u00f3s J.","year":"2024","unstructured":"J. Mart\u00ednez-Campreci\u00f3s, J. Espinosa-Pereiro, A. S\u00e1nchez-Montalv\u00e1, Update on the treatment of tuberculosis. Med. Clin. 163, 245\u2013252 (2024).","journal-title":"Med. Clin."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2024.119701"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2023.102670"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2022.2150449"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.88"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10989-022-10435-9"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2016.05.007"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929866530666230315113624"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000912"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2023.100954"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/424463"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2014.08.001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222111401"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2022.107968"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2023.108296"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.02.020"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-021-1955-4"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm2343"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05870-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-025-02774-x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.529594"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06775.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.032821-0"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02263-12"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.4A0713-363RR"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5386.121"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00337-22"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.4487"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1227191"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01488-1"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22147597"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/383829"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.09.012"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-017-3161-3"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.045112"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2021.110046"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/med.21429"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2020.11.020"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19113495"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.06.011"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.03.022"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abh2841"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)02479-6"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/resp.14887"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2023.116072"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1194923"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2016.01.031"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612824666180327162357"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2011.1203"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2023.122679"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2024.107661"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201807-1318OC"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3052"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20131199"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01328-24"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04597-22"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2011.09.009"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.15190"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep23089"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33516-1"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1093\/jimb\/kuae017"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9524-1"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00584-1"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2013.04.001"},{"key":"e_1_3_2_70_2","doi-asserted-by":"crossref","first-page":"e4966","DOI":"10.21769\/BioProtoc.4966","article-title":"Purification of Photorhabdus Virulence Cassette (PVC) protein complexes from Escherichia coli for artificial translocation of heterologous cargo proteins","volume":"14","author":"Wang Y.","year":"2024","unstructured":"Y. Wang, X. Zhang, X. Feng, X. Wang, Q. Jin, F. Jiang, Purification of Photorhabdus Virulence Cassette (PVC) protein complexes from Escherichia coli for artificial translocation of heterologous cargo proteins. Bio Protoc. 14, e4966 (2024).","journal-title":"Bio Protoc."},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.3302"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_554_18"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.TBTB2-0004-2015"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-38982-0"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.99282"},{"key":"e_1_3_2_76_2","first-page":"26","article-title":"Correlation between the kinetics of Th1, Th2 cells and pathology in a murine model of experimental pulmonary tuberculosis","volume":"89","author":"Hern\u00e1ndez-Pando R.","year":"1996","unstructured":"R. Hern\u00e1ndez-Pando, H. Orozcoe, A. Sampieri, L. Pav\u00f3n, C. Velasquillo, J. Larriva-Sahd, J. M. Alcocer, M. V. Madrid, Correlation between the kinetics of Th1, Th2 cells and pathology in a murine model of experimental pulmonary tuberculosis. Immunology 89, 26\u201333 (1996).","journal-title":"Immunology"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics12111071"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1038\/47042"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-023-03534-9"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts565"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa015"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.217"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0294236"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aee8463","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:01:56Z","timestamp":1790186516000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aee8463"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":86,"journal-issue":{"issue":"39","published-print":{"date-parts":[[2026,9,25]]}},"alternative-id":["10.1126\/sciadv.aee8463"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aee8463","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]},"assertion":[{"value":"2025-12-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaee8463"}}