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The conserved ER-phagy receptor, Atg40, contains a motif that binds to Atg8 and a reticulon homology domain that is needed for vacuolar\/lysosomal delivery. Cargo capture, however, requires the Atg40 binding partner Lst1\/SEC24C. To address whether lipids regulate cargo capture during ER-phagy, we analyzed autophagy in neutral lipid\u2013deficient cells. Unexpectedly, we found that Atg40 was delivered to the vacuole in autophagosomes without Lst1\/SEC24C or cargo in mutant cells. Lipidomic analysis revealed changes in the ratio of phosphatidylethanolamine to phosphatidylcholine in the neutral lipid\u2013deficient cells that are predicted to alter ER membrane bendability. 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