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In interphase, CENP-A and the CCAN assemble conserved shell-like structures that enclose a chromatin-poor central cavity. This cavity is occupied by the interphase-specific CENP-A chaperone complex, which promotes CENP-A assembly once per cell cycle. The presence of the chaperone complex, but not its CENP-A\u2013incorporating activity, is required to generate both the shell architecture and chromatin clearing. The CCAN scaffold CENP-C exhibits radial organization throughout the structure and is essential for its formation, primarily by recruiting the HJURP chaperone. Our findings broaden the role of the CENP-A chaperone machinery to include the structural organization of interphase vertebrate centromeres, independent of CENP-A deposition.<\/jats:p>","DOI":"10.1126\/sciadv.aef1579","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T17:58:47Z","timestamp":1788976727000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["The CENP-A chaperone complex spatially organizes centromeres"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0499-7333","authenticated-orcid":true,"given":"Hindol","family":"Gupta","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry &amp; Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5891-4241","authenticated-orcid":true,"given":"Julian","family":"Haase","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry &amp; 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