{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T18:58:28Z","timestamp":1785351508799,"version":"3.55.0"},"reference-count":48,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"31","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22225602"],"award-info":[{"award-number":["22225602"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22506197"],"award-info":[{"award-number":["22506197"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52293441"],"award-info":[{"award-number":["52293441"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"State Key Laboratory of Advanced Environmental Technology","award":["SKLAET2025-KD08"],"award-info":[{"award-number":["SKLAET2025-KD08"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,7,31]]},"abstract":"<jats:p>Molecular twist-related rotational dynamics at solid-liquid interfaces governs recognition, dissipation, and barrier crossing, yet direct label-free visualization of single-molecule torsion remains challenging. Here, we present twist-encoded magnetic plasmon interferometry (TEMPi), which converts nanorod twist near a metal film into a phase-encoded, orientation-resolved optical signal. Near-field coupling between a gold nanorod and its mirror image in the film excites a localized magnetic plasmon, enabling quantitative mapping of probe twist to interferometric phase. Wide-field, label-free operation in native buffers enables real-time tracking of rotational dynamics and interfacial conformational changes. TEMPi resolves three confinement-dependent rotational regimes, distinguishes effective dual- and single-contact protein anchoring, and localizes single binding sites on the probe through twist-dependent centroid shifts. Applied to DNA hybridization, it quantifies an effective ninefold increase in torsional stiffness and an order-of-magnitude faster twisting relaxation upon duplex formation. These results establish TEMPi as a general twist-resolved metrology for probing effective interfacial molecular mechanics and designing torsion-responsive interfaces in chemistry, biology, and nanotechnology.<\/jats:p>","DOI":"10.1126\/sciadv.aef5199","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T17:59:11Z","timestamp":1785347951000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Twist-encoded magnetic plasmon interferometry for label-free, orientation-resolved single-molecule imaging"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1640-4359","authenticated-orcid":true,"given":"Yi","family":"Sun","sequence":"first","affiliation":[{"name":"State Key Lab of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei 230026, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5067-7490","authenticated-orcid":true,"given":"Wen-Li","family":"Lv","sequence":"additional","affiliation":[{"name":"State Key Lab of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei 230026, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4746-7337","authenticated-orcid":true,"given":"Qi-Bing","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Lab of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei 230026, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7352-5993","authenticated-orcid":true,"given":"Gang","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Lab of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei 230026, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0493-8210","authenticated-orcid":true,"given":"Xian-Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Lab of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei 230026, China."},{"name":"Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1088\/1361-6633\/aa8a02"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41570-023-00540-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/C3CS60245A"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adf5509"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ads9687"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00221"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2020452118"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf581"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41646-3"},{"key":"e_1_3_2_11_2","first-page":"eabg3025","article-title":"Rotary biomolecular motor-powered supramolecular colloidal motor","volume":"9","author":"Liu J.","year":"2023","unstructured":"J. Liu, Y. Wu, Y. Li, L. Yang, H. Wu, Q. He, Rotary biomolecular motor-powered supramolecular colloidal motor. Sci. Adv. 9, eabg3025 (2023).","journal-title":"Sci. Adv."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202506464"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioelechem.2017.03.002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-022-01001-1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42719-z"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.4c03571"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00422"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42943-7"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202208299"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsphotonics.3c00937"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01001-9"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-032620-104637"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00021-6"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aea1572"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1063\/5.0131903"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl200135r"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl401656e"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1364\/OE.21.027438"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1364\/OL.39.001457"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.71.061902"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202407432"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c04987"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202001261"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01614-0"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep09803"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.631002"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.106.048103"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.109.188103"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.100.250602"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.79.011112"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.81.021103"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.12.4282"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymssp.2020.107321"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ins.2013.10.033"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2020.09.044"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30046-8"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34360-4"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl203299e"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18456-y"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aef5199","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T18:00:49Z","timestamp":1785348049000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aef5199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,31]]},"references-count":48,"journal-issue":{"issue":"31","published-print":{"date-parts":[[2026,7,31]]}},"alternative-id":["10.1126\/sciadv.aef5199"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aef5199","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,31]]},"assertion":[{"value":"2026-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaef5199"}}