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Adv."],"published-print":{"date-parts":[[2026,10,9]]},"abstract":"<jats:p>\n                    Extrachromosomal arrays are unique chromosome-like structures created from DNA injected into the\n                    <jats:italic toggle=\"yes\">Caenorhabditis elegans<\/jats:italic>\n                    germline. However, they are unstable unless integrated into a chromosome. Current methods for integration using x-rays or CRISPR can damage DNA and exhibit low efficiency. We demonstrate that the viral integrase PhiC31, which mediates nonmutagenic recombination between short\n                    <jats:italic toggle=\"yes\">attB<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">attP<\/jats:italic>\n                    sequences, can be used for extremely efficient and targeted integration of arrays. Arrays were integrated by PhIAT (PhiC31-mediated integration of arrays of transgenes) at\n                    <jats:italic toggle=\"yes\">attB<\/jats:italic>\n                    sites on three chromosomes, including at a fluorescent landing pad. Moreover, integrations can be inserted at any arbitrary site in the genome by simultaneously co-injecting Cas9 ribonucleoprotein, an\n                    <jats:italic toggle=\"yes\">attB<\/jats:italic>\n                    repair template, and the DNA components for the array\u2014thereby providing sites on all six chromosomes. Single injections can integrate arrays ranging in size from 1 to 18 megabases. PhIAT makes it practical to study genomes of other organisms in the nematode; one of our strains incorporates 65% of the yeast genome at a single site in the worm genome. PhIAT will accelerate a shift from unstable extrachromosomal arrays to direct integration of arrays in\n                    <jats:italic toggle=\"yes\">C. elegans<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aef6902","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:58:33Z","timestamp":1791395913000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Targeted integration of extrachromosomal arrays in\n                    <i>C. elegans<\/i>\n                    using PhiC31 integrase"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2353-4681","authenticated-orcid":true,"given":"Matthew S.","family":"Rich","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, United States."},{"name":"Howard Hughes Medical Institute, School of Biological Sciences, University of Utah, Salt Lake City, United States."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3333-7825","authenticated-orcid":true,"given":"Ryan","family":"Pellow","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, United States."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9809-4935","authenticated-orcid":true,"given":"Yichen","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, United States."},{"name":"Howard Hughes Medical Institute, School of Biological Sciences, University of Utah, Salt Lake City, United States."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Hefel","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, United States."},{"name":"Howard Hughes Medical Institute, School of Biological Sciences, University of Utah, Salt Lake City, United States."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6558-6194","authenticated-orcid":true,"given":"Ofer","family":"Rog","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, United States."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2978-8028","authenticated-orcid":true,"given":"Erik M.","family":"Jorgensen","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, United States."},{"name":"Howard Hughes Medical Institute, School of Biological Sciences, University of Utah, Salt Lake City, United States."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb04966.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.5.12.3484-3496.1985"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.09.016"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000817"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","unstructured":"C. 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