{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:40:56Z","timestamp":1792608056299,"version":"4.5.0"},"reference-count":57,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"43","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470913"],"award-info":[{"award-number":["32470913"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Laboratory of Herbivorous Livestock Breeding","award":["2026CXPT02003"],"award-info":[{"award-number":["2026CXPT02003"]}]},{"name":"NSF of Inner Mongolia Autonomous Region of China","award":["2023MS03007"],"award-info":[{"award-number":["2023MS03007"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,10,23]]},"abstract":"<jats:p>\n                    Nuclear envelope (NE) reassembly is essential for the zygote-to-totipotency transition in mammalian embryos. Beyond its classical roles in nuclear transport and maintenance of the RanGTP gradient, the function of RanGAP1 in embryonic NE reassembly has remained elusive. Here, we show that RanGAP1 localizes to the early embryonic NE, driving reassembly via a distinct structural mechanism. Trim-Away\u2013mediated RanGAP1 depletion causes two-cell stage arrest and severe NE defects, including nuclear pore complex (NPC) loss, failed reassembly, and aberrant \u201cfishnet\u201d membrane structures. Mechanistically, RanGAP1 establishes a structural paradigm for endoplasmic reticulum (ER)\u2013lamina coupling by recruiting Lamin A\/C, B1, and B2 to the ER for coassembly. The acidic domain and SUMO-1 modification at Lys\n                    <jats:sup>526<\/jats:sup>\n                    (K526) are essential for the NPC localization of RanGAP1. Thus, RanGAP1 orchestrates nuclear lamina (NL) assembly by targeting lamins to the ER. These findings uncover mechanisms of NE reassembly that are critical for early embryogenesis and provide insights into NE-related developmental disorders.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aef7931","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:01:42Z","timestamp":1792605702000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["RanGAP1 coordinates the ER-lamina interface to drive de novo nuclear envelope reassembly in mouse embryos"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7938-1891","authenticated-orcid":true,"given":"Xiao-Jie","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, Inner Mongolia 010070, 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