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Adv."],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:p>Recording and real-time imaging of promoter activities are critical for deciphering signaling cross-talk, but technologies for simultaneously capturing multiple transient events in living cells are lacking. Here, we design fluorescent protein\u2013based ticker tapes (FPTT) for multiplexed, scalable, longitudinal recording of single-cell physiological activities by integrating multispectral monomeric fluorescent proteins with self-assembling protein fibers. FPTT logged dose-dependent, reversible endogenous cFos transcriptional histories in hippocampal neurons at 3-hour resolution over 8 days. We engineered FPTT variants for human nuclear factor \u03baB (NF-\u03baB), Janus kinase\/signal transducer and activator of transcription 3 (STAT3), mechanistic target of rapamycin (mTOR), nuclear factor of activated T cells (NFAT), and adenosine 3\u2032,5\u2032-monophosphate (cAMP) signaling. This expanded toolset enabled quantification of cFos and NF-\u03baB cross-talk in neurons, tracking of STAT3\/cAMP dynamics during mouse liver injury, discovery of unexpected NFAT\/STAT3 cross-talk, and characterization of cell cycle\u2013dependent oscillating mTOR dynamics. Last, we achieved simultaneous analysis of four major pathways during T cell activation. FPTT provides a versatile platform to investigate transcriptional histories and signaling interplay, with broad applications in developmental biology and disease modeling.<\/jats:p>","DOI":"10.1126\/sciadv.aef9406","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:59:26Z","timestamp":1788371966000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Fluorescent protein ticker tape (FPTT): Multiplexed recording of transcriptional dynamics in living cells and in vivo"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1608-2322","authenticated-orcid":true,"given":"Ruizhao","family":"Wang","sequence":"first","affiliation":[{"name":"College of Life Sciences, Zhejiang University, Hangzhou, China."},{"name":"School of Life Sciences, Westlake University, Hangzhou, China."},{"name":"Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, 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