{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:54:14Z","timestamp":1792738454070,"version":"4.5.0"},"reference-count":49,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"29","funder":[{"DOI":"10.13039\/100018703","name":"European Innovation Council","doi-asserted-by":"publisher","award":["101046928"],"award-info":[{"award-number":["101046928"]}],"id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,7,17]]},"abstract":"<jats:p>The actin cytoskeleton in activated T cells undergoes rapid structural changes during the formation of an immunological synapse. Superresolution fluorescence microscopy provides excellent means to visualize such antibody-triggered changes. Here, we use single-molecule localization microscopy (SMLM) enabled by transparent polymer waveguide chips to resolve the filamentous-actin (F-actin) cytoskeleton in activated Jurkat T cells in comparison to nonactivated T cells across a large field of view. Transparent polymer waveguides enable a wide array of imaging modalities. In combination, these modalities reveal the structural differences between lamellipodial and ramified actin networks within the immunological synapse of activated T cells. SMLM images recorded by using narrow-width waveguide total internal reflection illumination resolve the double-stranded helical structure of actin filaments in activated Jurkat T cells. The average crossover length of the filaments is measured to be ~40 nanometers, which corroborates similar observations of isolated actin filaments by electron microscopy.<\/jats:p>","DOI":"10.1126\/sciadv.aeg3960","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T18:03:21Z","timestamp":1784138601000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Photonic waveguide chip\u2013based nanoscopy visualizes rearrangements of the cortical actin cytoskeleton in activated Jurkat T cells"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7471-4434","authenticated-orcid":true,"given":"Surjendu Bikash","family":"Dutta","sequence":"first","affiliation":[{"name":"Biomolecular Photonics, Faculty of Physics, University of Bielefeld, Bielefeld, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1128-6468","authenticated-orcid":true,"given":"Wolfgang","family":"H\u00fcbner","sequence":"additional","affiliation":[{"name":"Biomolecular Photonics, Faculty of Physics, University of Bielefeld, Bielefeld, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0520-6541","authenticated-orcid":true,"given":"Paul","family":"Goffing","sequence":"additional","affiliation":[{"name":"Biomolecular Photonics, Faculty of Physics, University of Bielefeld, Bielefeld, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5515-0706","authenticated-orcid":true,"given":"Anders Kokkvoll","family":"Engdahl","sequence":"additional","affiliation":[{"name":"Biomolecular Photonics, Faculty of Physics, University of Bielefeld, Bielefeld, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0392-0868","authenticated-orcid":true,"given":"Stefan","family":"Belle","sequence":"additional","affiliation":[{"name":"Applied Laser and Photonics Group, Aschaffenburg University of Applied Sciences, Aschaffenburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5592-3388","authenticated-orcid":true,"given":"Ralf","family":"Hellmann","sequence":"additional","affiliation":[{"name":"Applied Laser and Photonics Group, Aschaffenburg University of Applied Sciences, Aschaffenburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5338-1706","authenticated-orcid":true,"given":"Mark","family":"Sch\u00fcttpelz","sequence":"additional","affiliation":[{"name":"Biomolecular Photonics, Faculty of Physics, University of Bielefeld, Bielefeld, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9874-5008","authenticated-orcid":true,"given":"Francesco","family":"Dell\u2019Olio","sequence":"additional","affiliation":[{"name":"Micro Nano Sensor Group, Polytechnic University of Bari, Bari, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2348-7416","authenticated-orcid":true,"given":"Thomas","family":"Huser","sequence":"additional","affiliation":[{"name":"Biomolecular Photonics, Faculty of Physics, University of Bielefeld, Bielefeld, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.665519"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00112-1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1603032"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710258105"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2013.05.004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.232322"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1710751114"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e11-08-0731"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.676066"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E14-03-0830"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.01.038"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.02.069"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.71"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583514000146"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137395"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127344"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201002018"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2818.2000.00710.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.120345"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1364\/OL.19.000780"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04592"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00038-x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth929"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907866106"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsphotonics.5b00307"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12471"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-02057-w"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.080085"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100791"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3466"},{"key":"e_1_3_2_32_2","first-page":"e53749","article-title":"Cortical actin flow in T cells quantified by spatio-temporal image correlation spectroscopy of structured illumination microscopy data","volume":"2015","author":"Ashdown G.","year":"2015","unstructured":"G. Ashdown, E. Pand\u017ei\u0107, A. Cope, P. Wiseman, D. Owen, Cortical actin flow in T cells quantified by spatio-temporal image correlation spectroscopy of structured illumination microscopy data. J. Vis. Exp. 2015, e53749 (2015).","journal-title":"J. Vis. Exp."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28691-5"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e16-05-0330"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nphoton.2017.55"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41377-022-00731-w"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09247-1"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1364\/OE.27.006700"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1515\/nanoph-2022-0100"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/adom.202402783"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsphotonics.1c00914"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43246-021-00192-5"},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","unstructured":"A. K. Engdahl S. B. Dutta S. Belle J. Sch\u00fcrstedt K. Szafranska P. McCourt R. Hellmann T. Huser M. Sch\u00fcttpelz Dual-color single molecule localization microscopy on transparent polymer waveguide chips. bioRxiv 518375 [Preprint] (2022); https:\/\/doi.org\/10.1101\/2022.11.29.518375.","DOI":"10.1101\/2022.11.29.518375"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.336"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2448"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2023.168295"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1612719113"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu202"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200801518"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-0938-1"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aeg3960","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T18:03:50Z","timestamp":1784138630000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aeg3960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,17]]},"references-count":49,"journal-issue":{"issue":"29","published-print":{"date-parts":[[2026,7,17]]}},"alternative-id":["10.1126\/sciadv.aeg3960"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aeg3960","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,17]]},"assertion":[{"value":"2026-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaeg3960"}}