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Adv."],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:p>\n                    Chlorophylls are essential for photosynthesis, yet their heterologous production is a major challenge in synthetic biology due to pathway complexity and the diversion of resources away from essential cofactor biosynthesis. Here, we reconstruct the complete chlorophyll biosynthetic pathway in\n                    <jats:italic toggle=\"yes\">Saccharomyces cerevisiae<\/jats:italic>\n                    . By integrating 13 heterologous genes and deleting 3 endogenous genes, we engineered a yeast chassis capable of producing chlorophyll a at titers of 26\u00a0micrograms per gram of dry cell weight. This platform was further expanded with a ketocarotenoid biosynthetic module to produce echinenone and canthaxanthin, enabling the in vivo coassembly of two distinct pigment-protein complexes: the plant-derived water-soluble chlorophyll protein and the cyanobacterial orange carotenoid protein (Syn_OCP). Spectroscopic and biochemical analyses confirm that these yeast-assembled complexes retain native spectral and functional signatures, including Syn_OCP photoactivity, demonstrating high-fidelity pigment binding. Our work establishes a versatile eukaryotic platform for producing photosynthetic complexes, which could help open avenues for studying their assembly outside plants and for engineering light-driven metabolism in nonphotosynthetic eukaryotes.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeg6969","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:58:46Z","timestamp":1788544726000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["De novo coassembly of functional chlorophyll- and carotenoid-protein complexes in\n                    <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8212-1679","authenticated-orcid":true,"given":"Chenhao","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8457-819X","authenticated-orcid":true,"given":"Guangyu E.","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-008-9395-x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV120.006194"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaq1407"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.3c00237"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28908"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.02.015"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1987.tb09240.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","unstructured":"H. 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