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Adv."],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:p>\n                    Plant cells are connected by plasmodesmata (PD), membrane-lined channels that facilitate cell-to-cell transport. Forward genetic screens to uncover regulators of PD transport identified mutants with increased (\n                    <jats:italic toggle=\"yes\">ise1<\/jats:italic>\n                    to\n                    <jats:italic toggle=\"yes\">ise4<\/jats:italic>\n                    ) or decreased (\n                    <jats:italic toggle=\"yes\">dse1<\/jats:italic>\n                    ) PD trafficking during embryogenesis. Despite their opposite effects on PD transport, we found that the transcriptional profiles of\n                    <jats:italic toggle=\"yes\">dse1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">ise3<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ise4<\/jats:italic>\n                    were notably similar with one notable exception: the set of genes controlled by the conserved kinase TARGET OF RAPAMYCIN (TOR) and ABI5, a bZIP transcription factor that acts downstream of TOR. We then showed that the glucose-TOR-ABI5 signaling axis regulates PD transport by driving expression of PD-localized callose binding proteins (PDCBs), which are oppositely regulated in\n                    <jats:italic toggle=\"yes\">ise<\/jats:italic>\n                    versus\n                    <jats:italic toggle=\"yes\">dse<\/jats:italic>\n                    mutants and promote callose deposition at PD. Together, this study establishes a mechanism for metabolic regulation of cell-to-cell transport by TOR-ABI5-PDCB signaling.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeg9566","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:58:46Z","timestamp":1788544726000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Glucose-TOR-PDCB signaling restricts cell-to-cell communication through callose deposition"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8845-3300","authenticated-orcid":true,"given":"Michael","family":"Busche","sequence":"first","affiliation":[{"name":"Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9777-2132","authenticated-orcid":true,"given":"Anne M.","family":"Runkel","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7206-0059","authenticated-orcid":true,"given":"Min","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA."},{"name":"Chinese Education Ministry\u2019s Key Laboratory of Western Resources and Modern Biotechnology, Key Laboratory of Biotechnology Shaanxi Province, College of Life Sciences, Northwest University, Xi\u2019an 710069, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7768-9457","authenticated-orcid":true,"given":"Katherine A.","family":"Klimpel","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7868-6359","authenticated-orcid":true,"given":"Sannidhi","family":"Menon","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6303-9314","authenticated-orcid":true,"given":"Katherine M.","family":"Lund","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4585-8561","authenticated-orcid":true,"given":"Kyle","family":"Koch","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Snigdha","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA."},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA."},{"name":"Plant Gene Expression Center, USDA Agricultural Research Service, Albany, CA 94710, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6319-4303","authenticated-orcid":true,"given":"Erin R.","family":"Alberts","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan E.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6454-7721","authenticated-orcid":true,"given":"M. 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