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Adv."],"published-print":{"date-parts":[[2026,10,23]]},"abstract":"<jats:p>\n                    Malaria pathogenesis and clinical symptoms arise from blood-stage replication of\n                    <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                    parasites, which depends on rapid invasion of red blood cells by merozoites. Despite the central importance of this stage, direct functional studies of\n                    <jats:italic toggle=\"yes\">Plasmodium vivax<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                    ) merozoites remain limited by the lack of continuous in vitro culture and restricted access to viable, invasion-competent merozoites from clinical isolates. Consequently, key aspects of\n                    <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                    merozoite biology and immune recognition remain poorly defined. Here, we establish a robust platform enabling synchronized release and high-yield isolation of invasion-competent\n                    <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                    merozoites from ex vivo cultures. Reversible inhibition of parasite egress using the PKG inhibitor ML10 enables synchronization at the segmented schizont stage, followed by rapid mechanical release of merozoites using a liposome-based extrusion approach. This workflow yields highly pure merozoites with recovery rates exceeding 75% while preserving structural integrity and invasive capacity. Leveraging this platform, we demonstrate that antibody-opsonized\n                    <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                    merozoites activate human natural killer cells, revealing functional antibody-dependent immune responses.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeh3417","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:01:42Z","timestamp":1792605702000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Accessing invasion-competent\n                    <i>Plasmodium vivax<\/i>\n                    merozoites reveals antibody-dependent NK cell activation"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7684-4689","authenticated-orcid":true,"given":"Jeremy","family":"Salvador","sequence":"first","affiliation":[{"name":"Malaria Research Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Guselli","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7249-0114","authenticated-orcid":true,"given":"Romminea","family":"Yeat","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sokchea","family":"Lay","sequence":"additional","affiliation":[{"name":"Immunology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2228-2068","authenticated-orcid":true,"given":"Titsophantharoth","family":"Thol","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4176-4731","authenticated-orcid":true,"given":"Nisa","family":"Ya","sequence":"additional","affiliation":[{"name":"Immunology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1578-455X","authenticated-orcid":true,"given":"Lionel Brice","family":"Feufack-Donfack","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8341-1209","authenticated-orcid":true,"given":"Sievleang","family":"Heng","sequence":"additional","affiliation":[{"name":"Immunology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5066-2641","authenticated-orcid":true,"given":"Candice","family":"Bohaud","sequence":"additional","affiliation":[{"name":"Immunology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Orban","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4563-3852","authenticated-orcid":true,"given":"L\u00e9a","family":"Baldor","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6182-4010","authenticated-orcid":true,"given":"Baura","family":"Tat","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-9865","authenticated-orcid":true,"given":"Chetan E.","family":"Chitnis","sequence":"additional","affiliation":[{"name":"Malaria Parasite Biology and Vaccines Unit, INSERM UMR 1347 ParasitInnov, Universit\u00e9 Paris Cit\u00e9, Institut Pasteur, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8911-1502","authenticated-orcid":true,"given":"Tineke","family":"Cantaert","sequence":"additional","affiliation":[{"name":"Immunology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia."},{"name":"Ecology and Emergence of Arthropod-borne 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