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Adv."],"published-print":{"date-parts":[[2026,10,16]]},"abstract":"<jats:p>\n                    In plants, TONSOKU (TSK) regulates heterochromatic DNA replication and genome stability by preferentially binding histone H3.1 over its close homolog, histone H3.3. The structure of this interaction showed that a shallow pocket in TSK\u2019s poly-tetratricopeptide repeat (TPR\n                    <jats:sup>TSK<\/jats:sup>\n                    ) domain discriminates between the variants but did not report the importance of additional interactions underlying its binding to histone H3.1. In this study, we identify additional binding pockets that are important for histone H3.1 binding and TSK-like (TONSL)\u2013dependent DNA repair. Consistently, sponastrime dysplasia-linked mutations in the TPR domain of TONSL impair histone H3.1 binding and fail to rescue TONSL\u2019s DNA repair function in human cells. TPR\n                    <jats:sup>TSK<\/jats:sup>\n                    binding is highly regulated by posttranslational modifications (PTMs) on histone H3.1. Unexpectedly, TPR\n                    <jats:sup>TSK<\/jats:sup>\n                    contains two additional pockets that cooperate to recognize mono-methylated K4 and acetylated K27 on histone H3.1, two modifications associated with transcriptional regulatory elements like enhancers and promoters. Overall, our findings reveal that interactions between histone H3.1 and the TPR domains of TSK\/TONSL are regulated by multiple PTMs and are functionally relevant for their DNA repair functions.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeh3827","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:58:53Z","timestamp":1792173533000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["TONSL\/TONSOKU binding to histone H3.1 is required for DNA repair"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6049-1492","authenticated-orcid":true,"given":"Hossein","family":"Davarinejad","sequence":"first","affiliation":[{"name":"Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, 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