{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:51:12Z","timestamp":1791571872184,"version":"4.3.4"},"reference-count":62,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"41","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["TL1TR003019"],"award-info":[{"award-number":["TL1TR003019"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007388"],"award-info":[{"award-number":["T32GM007388"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR066070"],"award-info":[{"award-number":["R01AR066070"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR068320"],"award-info":[{"award-number":["R01AR068320"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD105009"],"award-info":[{"award-number":["R01HD105009"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100031445","name":"New Jersey Commission on Cancer Research","doi-asserted-by":"publisher","award":["COCR24PDF018"],"award-info":[{"award-number":["COCR24PDF018"]}],"id":[{"id":"10.13039\/100031445","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,10,9]]},"abstract":"<jats:p>Planar cell polarity (PCP) in epithelia is characterized by the polarized distribution of two opposing, membrane-associated PCP complexes across cell junctions. Transmembrane components of the PCP complex bridge cell junctions and organize into punctate, intercellular assemblies that exhibit a high degree of stability. Here, we define the contributions of the cytoplasmic PCP protein, Dishevelled (Dvl), in the submicrometer scale organization and stability of PCP complexes. Using endogenously tagged fluorescent PCP reporters in the embryonic mouse epidermis, we quantify PCP protein mobility and clustering during polarization. We find that as transmembrane proteins immobilize into puncta, Dvl2\/3 coaccumulates with its transmembrane partner Frizzled 6 in a polarized manner and stabilizes clusters of PCP complexes. We identify a previously unknown function for the oligomerizing Dishevelled\/Axin (DIX) domain of Dvl3, typically associated with Wnt signaling, in Dvl3 asymmetric localization. These observations underscore a role for Dvl oligomerization in assembly and stabilization of asymmetric PCP puncta.<\/jats:p>","DOI":"10.1126\/sciadv.aeh6920","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:06:59Z","timestamp":1791569219000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Dishevelled-mediated clustering stabilizes Frizzled6 and Vangl2 to establish planar cell polarity in the mammalian skin"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-8110","authenticated-orcid":true,"given":"Parijat","family":"Sil","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4810-7897","authenticated-orcid":true,"given":"Brandon","family":"Trejo","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6993-5312","authenticated-orcid":true,"given":"Katherine A.","family":"Little","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5464-259X","authenticated-orcid":true,"given":"Danelle","family":"Devenport","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.11"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.054080"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201408039"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20190404"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1784"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.199695"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.ads5704"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62097"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00715-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.03.018"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.08.016"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005259"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.10.039"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-64563-z"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","unstructured":"S. B. Nissen A. T. Weiner K. Suyama P. S. Bosch M. Yu S. Song Y. Gu A. R. Dunn J. D. Axelrod Cluster assembly dynamics drive fidelity of planar cell polarity polarization. bioRxiv 2024.10.21.619498 [Preprint] (2025). https:\/\/doi.org\/10.1101\/2024.10.21.619498.","DOI":"10.1101\/2024.10.21.619498"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394592-1.00007-7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2018.03.004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2015.11.027"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.195685"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81226-X"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.890501"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.16.2610"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/35011077"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1247"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2014.08.006"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2017.10.008"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.55015"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.13.2581"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02347"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402802101"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.10.027"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2017.06.003"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0082-7"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000259"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2020"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.06.023"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.02.078"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.1064907"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.06.030"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.11.021"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202205069"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2167"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-052118-115534"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.139063"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2024.12.012"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-042910-155238"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad9964"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.72588"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.07.037"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108121"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.08.004"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00336-1"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00167-3"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.6.2228-2238.2000"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-57658-0"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/161.2.747"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.06.022"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.15.8551"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-01018-x"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6371-3_13"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.07.042"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-060-7_14"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aeh6920","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:07:24Z","timestamp":1791569244000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aeh6920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,9]]},"references-count":62,"journal-issue":{"issue":"41","published-print":{"date-parts":[[2026,10,9]]}},"alternative-id":["10.1126\/sciadv.aeh6920"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aeh6920","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,9]]},"assertion":[{"value":"2026-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaeh6920"}}