{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:26:32Z","timestamp":1792787192042,"version":"4.5.0"},"reference-count":34,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"42","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI175038"],"award-info":[{"award-number":["R01AI175038"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K08AI159384"],"award-info":[{"award-number":["K08AI159384"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI195492"],"award-info":[{"award-number":["R01AI195492"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI195492"],"award-info":[{"award-number":["R01AI195492"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R44AI167078"],"award-info":[{"award-number":["R44AI167078"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Pediatrics, Washington University School of Medicine","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"name":"Gates Foundation","award":["INV-088225"],"award-info":[{"award-number":["INV-088225"]}],"id":[{"id":"https:\/\/ror.org\/0456r8d26","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,10,16]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    is the leading cause of neonatal sepsis in low- and middle-income countries and the foremost cause of infectious deaths in young children.\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    isolates are becoming increasingly resistant to antibiotics, creating a great need for additional therapies or preventative strategies. Maternal vaccination serves as an attractive approach to protect from\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    neonatal sepsis. Herein, we report a murine model of maternal immunization followed by pup challenge to evaluate putative\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    vaccines. Dams were immunized with capsular polysaccharide bioconjugate vaccines at mating and boosted with two different immunization schedules throughout the gestational period. After parturition, offspring were tested for antigen-specific antibody levels and later challenged with a lethal dose of\n                    <jats:italic toggle=\"yes\">K. pneumoniae.<\/jats:italic>\n                    Pups born to dams immunized with K1- and K2-conjugate vaccines demonstrated significant protection from sepsis relative to control pups. Antibodies transferred from vaccinated dams persisted and remained protective in pups for at least six weeks after birth. Collectively these results demonstrate a feasible and reproducible murine model of maternal immunization verifying the efficacy of bioconjugate vaccines in protecting against lethal neonatal\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aeh8222","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:59:24Z","timestamp":1792000764000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Maternal vaccination with capsular polysaccharide bioconjugates protects against murine\n                    <i>Klebsiella pneumoniae<\/i>\n                    neonatal sepsis"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6993-6320","authenticated-orcid":true,"given":"Paeton L.","family":"Wantuch","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6388-4868","authenticated-orcid":true,"given":"Jessica","family":"Tung","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lloyd S.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Omniose, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5042-3568","authenticated-orcid":true,"given":"Isra","family":"Darwech","sequence":"additional","affiliation":[{"name":"Omniose, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cory J.","family":"Knoot","sequence":"additional","affiliation":[{"name":"Omniose, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2158-3546","authenticated-orcid":true,"given":"Christian M.","family":"Harding","sequence":"additional","affiliation":[{"name":"Omniose, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1568-7733","authenticated-orcid":true,"given":"Drew J.","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110, USA."},{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA."},{"name":"Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, MO 63110, USA."},{"name":"Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1081-772X","authenticated-orcid":true,"given":"David A.","family":"Rosen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110, USA."},{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00078-15"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00870-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2023.22494"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1004233"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1004239"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2024.02.072"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011367"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-025-01314-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2024.126536"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1907833116"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02994-19"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz062"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2016.1265932"},{"key":"e_1_3_2_15_2","first-page":"e52018","article-title":"Non-invasive model of neuropathogenic Escherichia coli infection in the neonatal rat","author":"Dalgakiran F.","year":"2014","unstructured":"F. Dalgakiran, L. A. Witcomb, A. J. McCarthy, G. M. Birchenough, P. W. Taylor, Non-invasive model of neuropathogenic Escherichia coli infection in the neonatal rat. J. Vis. Exp., e52018 (2014).","journal-title":"J. Vis. Exp."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-46775-x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00697-25"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-33619-x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-08258-7"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiae097"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00538-25"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00176-25"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaf600"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1004879"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2023.07.023"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.adg5562"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20200325"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/jimmun\/vkaf235"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00776-18"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017288"},{"key":"e_1_3_2_32_2","unstructured":"B. Bushnell BBMap: A fast accurate splice-aware aligner. Lawrence Berkeley National Laboratory. LBNL Report no. LBNL-7065E; retrieved from https:\/\/escholarship.org\/uc\/item\/1h3515gn (2014)."},{"key":"e_1_3_2_33_2","unstructured":"S. Andrews FastQC: A quality control tool for high throughput sequence data [Online]. Available online at: https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01688-w"},{"key":"e_1_3_2_35_2","first-page":"56921","article-title":"Breast milk enhances growth of enteroids: An ex vivo model of cell proliferation","author":"Lanik W. E.","year":"2018","unstructured":"W. E. Lanik, L. Xu, C. J. Luke, E. Z. Hu, P. Agrawal, V. S. Liu, R. Kumar, A. M. Bolock, C. Ma, M. Good, Breast milk enhances growth of enteroids: An ex vivo model of cell proliferation. J. Vis. Exp., 56921 (2018).","journal-title":"J. Vis. Exp."}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aeh8222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:00:36Z","timestamp":1792000836000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aeh8222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,16]]},"references-count":34,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2026,10,16]]}},"alternative-id":["10.1126\/sciadv.aeh8222"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aeh8222","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,16]]},"assertion":[{"value":"2026-04-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eaeh8222"}}