{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:26:33Z","timestamp":1792787193279,"version":"4.5.0"},"reference-count":61,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"38","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21 AI 180600"],"award-info":[{"award-number":["R21 AI 180600"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Michigan State University Roudolph Hugh Endowment","id":[{"id":"https:\/\/ror.org\/05hs6h993","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2026,9,18]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Campylobacter jejuni<\/jats:italic>\n                    is a major enteric pathogen whose ability to grow in the inflamed gut remains poorly understood, limiting the development of effective therapeutic strategies. Our prior study in ferrets suggested that intestinal inflammation promotes\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    expansion during infection. However, conventional mice are naturally resistant to\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    colonization unless the microbiota or host inflammatory pathways are altered. To directly investigate the role of inflammation in\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    infection, transient colitis was induced in conventional mice using short-term dextran sodium sulfate (DSS) treatment. DSS-mediated inflammation disrupted colonization resistance and enabled rapid\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    growth in the colon within three days of infection, accompanied by aggravated intestinal inflammation. Microbiota analysis revealed enrichment of mucin-degrading bacteria and depletion of taxa associated with short-chain fatty acid and indole production. Metabolomic profiling further demonstrated significantly reduced colonic indole levels in DSS-treated and infected mice. In vitro studies showed that physiological concentrations of indole inhibited\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    growth and downregulated genes involved in major energy-generating pathways, including nitrate respiration (\n                    <jats:italic toggle=\"yes\">napA<\/jats:italic>\n                    ), aerobic respiration (\n                    <jats:italic toggle=\"yes\">ccoN<\/jats:italic>\n                    ), lactate utilization (\n                    <jats:italic toggle=\"yes\">lctP<\/jats:italic>\n                    ), and the acetate switch (\n                    <jats:italic toggle=\"yes\">ackA\/ptaA<\/jats:italic>\n                    ). Consistent with these findings, mutations in these pathways reduced bacterial fitness in DSS-treated mice, highlighting their importance for colonization in the inflamed intestine. Furthermore, administration of indole or the indole-producing probiotic\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    Nissle 1917 significantly decreased\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    colonization in vivo. Collectively, these findings demonstrate that intestinal inflammation facilitates\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    colonization while microbiota-derived metabolites, particularly indole, play a critical role in suppressing pathogen growth and pathogenicity.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.aei6934","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:02:56Z","timestamp":1789581776000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Microbiota-derived indole limits\n                    <i>Campylobacter jejuni<\/i>\n                    colonization by inhibiting respiration and metabolism"],"prefix":"10.1126","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7809-5324","authenticated-orcid":true,"given":"Ritam","family":"Sinha","sequence":"first","affiliation":[{"name":"Department of Microbiology Genetics &amp; Immunology, Michigan State University, East Lansing, MI 48824."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barsha","family":"Bhattarai","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Northern Illinois University, DeKalb IL-60115."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3485-2434","authenticated-orcid":true,"given":"Cristina Kraemer","family":"Zimpel","sequence":"additional","affiliation":[{"name":"Department of Microbiology Genetics &amp; Immunology, Michigan State University, East Lansing, MI 48824."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6234-2789","authenticated-orcid":true,"given":"Elizabeth","family":"Ottosen","sequence":"additional","affiliation":[{"name":"Department of Microbiology Genetics &amp; Immunology, Michigan State University, East Lansing, MI 48824."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhiannon M.","family":"LeVeque","sequence":"additional","affiliation":[{"name":"Department of Microbiology Genetics &amp; Immunology, Michigan State University, East Lansing, MI 48824."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0283-7459","authenticated-orcid":true,"given":"Pallavi","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Northern Illinois University, DeKalb IL-60115."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3617-7011","authenticated-orcid":true,"given":"Victor J.","family":"DiRita","sequence":"additional","affiliation":[{"name":"Department of Microbiology Genetics &amp; Immunology, Michigan State University, East Lansing, MI 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