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Strikingly, CD8 memory T cells isolated from nonlymphoid tissues exhibited effector levels of lytic activity directly ex vivo, in contrast to their splenic counterparts. These results point to the existence of a population of extralymphoid effector memory T cells poised for immediate response to infection.<\/jats:p>","DOI":"10.1126\/science.1058867","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:52:08Z","timestamp":1027749128000},"page":"2413-2417","source":"Crossref","is-referenced-by-count":1519,"title":["Preferential Localization of Effector Memory Cells in Nonlymphoid Tissue"],"prefix":"10.1126","volume":"291","author":[{"given":"David","family":"Masopust","sequence":"first","affiliation":[{"name":"Division of Immunology, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaiva","family":"Vezys","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda L.","family":"Marzo","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo","family":"Lefranc\u0327ois","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.14.1.207"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.275"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1155\/1992\/57057"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.4.1367"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80469-0"},{"key":"e_1_3_1_7_2","doi-asserted-by":"crossref","unstructured":"K. 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U.S.A. 93 9716 (1996)."},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/348213a0"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5284.94"},{"key":"e_1_3_1_22_2","unstructured":"For production of MHC class I tetramers bacteria-produced H-2K b containing the BirA-dependent biotinylation substrate sequence was folded in the presence of human \u03b22-microglobulin and the VSV-N peptide. Biotinylation was performed with biotin-protein ligase (Avidity Denver CO). Tetramers were then produced from biotinylated high-performance liquid chromatography\u2013purified monomers by addition of streptavidin-allophycocyanin (APC; Molecular Probes Eugene OR)."},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.6.2976"},{"key":"e_1_3_1_24_2","doi-asserted-by":"crossref","unstructured":"C. Pope et al. J. 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Lefran\u00e7ois unpublished data."},{"key":"e_1_3_1_29_2","unstructured":"Supplementary data are available at Science Online (www.sciencemag.org\/cgi\/content\/full\/291\/5512\/2413\/DC1)."},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.4.2348"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.5.2103"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13812"},{"key":"e_1_3_1_33_2","doi-asserted-by":"crossref","unstructured":"E. J. Usherwood et al. J. Virol. 73 7278 (1999).","DOI":"10.1128\/JVI.73.9.7278-7286.1999"},{"key":"e_1_3_1_34_2","doi-asserted-by":"crossref","unstructured":"K. Murali-Krishna et al. Science 286 1377 (1999).","DOI":"10.1126\/science.286.5443.1377"},{"key":"e_1_3_1_35_2","unstructured":"Before tissue removal mice were perfused with phosphate-buffered saline and heparin (75 U\/ml). IEL and LP cells were isolated as described (9). Lung tissue was minced and incubated with stirring at 37\u00b0C for 30 min in Hanks' balanced salt solution (HBSS) with 1.3 mM EDTA followed by treatment at 37\u00b0C for 1 hour with collagenase (150 U\/ml; 17018-037 Gibco-BRL) in RPMI with 5% fetal calf serum. The resulting suspension was pelleted by centrifugation resuspended in 44% Percoll (Pharmacia) layered on 67.5% Percoll and centrifuged at 600 g. Cells at the gradient interface were harvested and washed extensively before use. Liver tissue was mashed through a 70-\u03bcm strainer in HBSS with 2% fetal calf serum and 10 mM Hepes. The resulting suspension was centrifuged and the pellet resuspended in a solution of 35% Percoll and heparin (200 U\/ml) and centrifuged at 600 g. Cells in the resulting pellet were treated with tris\u2013ammonium chloride to remove red blood cells and then washed extensively before use."},{"key":"e_1_3_1_36_2","unstructured":"We thank J. Altman for modified H-2K b cDNA and E. Pamer for \u03b22-microglobulin constructs. Supported by U.S. Public Health Service (USPHS) grants DK45260 and AI41576 by USPHS training grant T32-AI07080 (D.M. and V.V.) and by a collaborative grant from the Edward Jenner Institute for Vaccine Research and a C. J. 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