{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T23:22:58Z","timestamp":1781047378101,"version":"3.54.1"},"reference-count":28,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5529","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2001,7,20]]},"abstract":"<jats:p>\n                    Little is known about the functional significance of spontaneous miniature synaptic potentials, which are the result of vesicular exocytosis at nerve terminals. Here, by using\n                    <jats:italic>Drosophila<\/jats:italic>\n                    mutants with specific defects in presynaptic function, we found that glutamate receptors clustered normally at neuromuscular junctions of mutants that retained spontaneous transmitter secretion but had lost the ability to release transmitter in response to action potentials. In contrast, receptor clustering was defective in mutants in which both spontaneous and evoked vesicle exocytosis were absent. Thus, spontaneous vesicle exocytosis appears to be tightly linked to the clustering of glutamate receptors during development.\n                  <\/jats:p>","DOI":"10.1126\/science.1061270","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:39:52Z","timestamp":1027748392000},"page":"514-517","source":"Crossref","is-referenced-by-count":107,"title":["Absence of Junctional Glutamate Receptor Clusters in\n                    <i>Drosophila<\/i>\n                    Mutants Lacking Spontaneous Transmitter Release"],"prefix":"10.1126","volume":"293","author":[{"given":"Minoru","family":"Saitoe","sequence":"first","affiliation":[{"name":"Institute for Behavioral Sciences, Gunma University School of Medicine, Maebashi, Gunma 371\u20138511, Japan."},{"name":"Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183\u20138526, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas L.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Stanford University Medical Center, Stanford, CA 94305, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy A.","family":"Umbach","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Pharmacology, School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron B.","family":"Gundersen","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Pharmacology, School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Kidokoro","sequence":"additional","affiliation":[{"name":"Institute for Behavioral Sciences, Gunma University School of Medicine, Maebashi, Gunma 371\u20138511, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1113\/jphysiol.1952.sp004735","volume":"117","author":"Fatt P.","year":"1952","unstructured":"Fatt P., Katz B., J. Physiol. 117, 109 (1952).","journal-title":"J. Physiol."},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/264787a0"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1977.sp011880"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/361350a0"},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","unstructured":"D. L. Deitcher et al. J. Neurosci. 18 2028 (1998).","DOI":"10.1523\/JNEUROSCI.18-06-02028.1998"},{"key":"e_1_3_1_7_2","doi-asserted-by":"crossref","unstructured":"M. Yoshihara et al. J. Neurosci. 19 2432 (1999).","DOI":"10.1523\/JNEUROSCI.19-07-02432.1999"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(94)90255-0"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90154-X"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.96.6.1517"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-0102(94)90137-6"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-12-07905.1995"},{"key":"e_1_3_1_13_2","unstructured":"Ventral longitudinal muscles (muscle 6 or 7 in the A4 segment) of embryos or newly hatched larvae at 22 hours after fertilization were used (10 11). Synaptic currents were recorded at \u221265 mV with the whole-cell patch-clamp technique. The ionic composition of the internal solution was as follows: KCl 158 mM; EGTA 5 mM; Hepes-NaOH 10 mM; and adenosine triphosphate 2 mM (pH 7.1). For measuring evoked synaptic currents the 0.5 mM Ca 2+ external solution was used which had the following ionic composition: NaCl 140 mM; KCl 2 mM; MgCl 2 6 mM; CaCl 2 0.5 mM; and Hepes-NaOH 5 mM (pH 7.1). To measure mESCs we added 1 \u03bcM TTX to the 0.5 mM Ca 2+ external solution to block Na + action potentials. To evoke ESCs we placed the tip of a microelectrode in the central nervous system (CNS) and positive pulses of 2 ms and about 2 \u03bcA were delivered."},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-11-04924.1993"},{"key":"e_1_3_1_15_2","unstructured":"Supplementary material is available on Science Online at www.sciencemag.org\/cgi\/content\/full\/293\/5529\/514\/DC1."},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.8.2700"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90073-Z"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-08-03023.1999"},{"key":"e_1_3_1_19_2","unstructured":"J. Morr B. Schmidt H. Betz C. Schuster personal communication."},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.4.861"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/144.4.1713"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-09-03233.1998"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.8480"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-01-00144.1993"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1990.055.01.041"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00197-5"},{"key":"e_1_3_1_27_2","doi-asserted-by":"crossref","unstructured":"R. J. O'Brien et al. Neuron 23 309 (1999).","DOI":"10.1016\/S0896-6273(00)80782-5"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5454.864"},{"key":"e_1_3_1_29_2","unstructured":"We thank A. Nose for the G14-GAL4 enhancer trap line; A. DiAntonio for the myc-tagged GluRIIB line; J. Morr B. Schmidt H. Betz and C. Schuster for the AS5 antibody; and Y. Kubo (Physiology) at Tokyo Medical and Dental University H. Okado and S. Ohsako at Tokyo Metropolitan Institute for Neuroscience and A. Grinnell (Physiology) and B. Howard (Biological Chemistry) at the University of California Los Angeles for critical reading of the manuscript. Supported by a grant-in-aid from the Ministry of Education Science Sports and Culture of Japan (M.S and Y.K.) the Brain Science Foundation (M.S.) and by NIH (J.A.U)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1061270","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T16:23:15Z","timestamp":1704817395000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1061270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7,20]]},"references-count":28,"journal-issue":{"issue":"5529","published-print":{"date-parts":[[2001,7,20]]}},"alternative-id":["10.1126\/science.1061270"],"URL":"https:\/\/doi.org\/10.1126\/science.1061270","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1000165.5603","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7,20]]}}}