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This methodology allowed gene function to be characterized in a comprehensive, defined set of conditionally growth-defective\/lethal isogenic strains. Quantitative titration of the conditional growth effect was performed either in bacterial culture or in an animal model of infection. This genomic strategy offers an approach to the identification of staphylococcal gene products that could serve as targets for antibiotic discovery.\n                  <\/jats:p>","DOI":"10.1126\/science.1063566","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:39:52Z","timestamp":1027748392000},"page":"2266-2269","source":"Crossref","is-referenced-by-count":373,"title":["Identification of Critical Staphylococcal Genes Using Conditional Phenotypes Generated by Antisense RNA"],"prefix":"10.1126","volume":"293","author":[{"given":"Yinduo","family":"Ji","sequence":"first","affiliation":[{"name":"Department of Microbiology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie F.","family":"Van","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Horn","sequence":"additional","affiliation":[{"name":"Genetics Research, GlaxoSmithKline Pharmaceuticals Research and Development, Collegeville, PA 19426, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Warren","sequence":"additional","affiliation":[{"name":"Genetics Research, GlaxoSmithKline Pharmaceuticals Research and Development, Collegeville, PA 19426, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Woodnutt","sequence":"additional","affiliation":[{"name":"Department of Microbiology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin K. 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After addition of template DNA the PCR mixture was denatured at 94\u00b0C for 2 min followed by the thermal cycle (30 s at 94\u00b0C 30 s at 51\u00b0C and 1.5 min at 72\u00b0C)."},{"key":"e_1_3_1_27_2","unstructured":"DNA sequences of all antisense segments contained in strains listed in tables are available upon request and identification numbers of unknown genes can be found on Web table 2 (24)."},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_1_29_2","unstructured":"Supported in part by the U.S. Defense Advanced Research Projects Agency (N65236-97-1-5810). We thank A. Marra and D. 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