{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:44:28Z","timestamp":1791895468404,"version":"4.3.4"},"reference-count":24,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5546","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2001,11,16]]},"abstract":"<jats:p>\n                    Proteins are transported from the endoplasmic reticulum (ER) in vesicles formed by coat protein complex II (COPII). Soluble secretory proteins are thought to leave the ER in these vesicles by \u201cbulk flow\u201d or through recognition by hypothetical shuttling receptors. We found that Erv29p, a conserved transmembrane protein, was directly required for packaging glycosylated pro-\u03b1-factor (gp\u03b1f) into COPII vesicles in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    . Further, an Erv29p-gp\u03b1f complex was isolated from ER-derived transport vesicles. In vivo, export of gp\u03b1f from the ER was saturable and depended on the expression level of Erv29p. These results indicate that membrane receptors can link soluble cargo proteins to the COPII coat.\n                  <\/jats:p>","DOI":"10.1126\/science.1065224","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:47:15Z","timestamp":1027748835000},"page":"1528-1531","source":"Crossref","is-referenced-by-count":187,"title":["Role of Erv29p in Collecting Soluble Secretory Proteins into ER-Derived Transport Vesicles"],"prefix":"10.1126","volume":"294","author":[{"given":"William J.","family":"Belden","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Barlowe","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0092-8674(00)81006-5","volume":"98","author":"Warren G.","year":"1999","unstructured":"Warren G., Mellman I., Cell 98, 125 (1999).","journal-title":"Cell"},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/34438","volume":"391","author":"Kuehn M. J.","year":"1998","unstructured":"Kuehn M. J., Herrmann J. M., Schekman R., Nature 391, 187 (1998).","journal-title":"Nature"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5377.698"},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/0092-8674(94)90359-X","volume":"76","author":"Balch W. E.","year":"1994","unstructured":"Balch W. E., McCaffery J. M., Plutner H., Farquhar M. G., Cell 76, 841 (1994).","journal-title":"Cell"},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0092-8674(00)80608-X","volume":"98","author":"Martinez-Menarguez J. A.","year":"1999","unstructured":"Martinez-Menarguez J. A., Geuze H. J., Slot J. W., Klumperman J., Cell 98, 81 (1999).","journal-title":"Cell"},{"key":"e_1_3_1_7_2","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1083\/jcb.148.5.925","volume":"148","author":"Muniz M.","year":"2000","unstructured":"Muniz M., Nuoffer C., Hauri H-P., Riezman H., J. Cell Biol. 148, 925 (2000).","journal-title":"J. Cell Biol."},{"key":"e_1_3_1_8_2","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/0092-8674(94)90138-4","volume":"77","author":"Barlowe C.","year":"1994","unstructured":"Barlowe C., et al., Cell 77, 895 (1994).","journal-title":"Cell"},{"key":"e_1_3_1_9_2","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1083\/jcb.152.3.503","volume":"152","author":"Otte S.","year":"2001","unstructured":"Otte S., et al., J. Cell Biol. 152, 503 (2001).","journal-title":"J. Cell Biol."},{"key":"e_1_3_1_10_2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.3109\/09687689509027508","volume":"12","author":"Reeves J. 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Cell Biol."},{"key":"e_1_3_1_13_2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/0092-8674(84)90224-1","volume":"36","author":"Julius D.","year":"1984","unstructured":"Julius D., Schekman R., Thorner J., Cell 36, 309 (1984).","journal-title":"Cell"},{"key":"e_1_3_1_14_2","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0092-8674(82)90241-0","volume":"30","author":"Stevens T.","year":"1982","unstructured":"Stevens T., Esmon B., Schekman R., Cell 30, 439 (1982).","journal-title":"Cell"},{"key":"e_1_3_1_15_2","unstructured":"W. J. Belden C. Barlowe unpublished data."},{"key":"e_1_3_1_16_2","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1016\/0092-8674(95)90144-2","volume":"83","author":"Bednarek S. Y.","year":"1995","unstructured":"Bednarek S. Y., et al., Cell 83, 1183 (1995).","journal-title":"Cell"},{"key":"e_1_3_1_17_2","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/14020","volume":"1","author":"Appenzeller C.","year":"1999","unstructured":"Appenzeller C., Andersson H., Kappeler F., Hauri H. P., Nature Cell Biol. 1, 330 (1999).","journal-title":"Nature Cell Biol."},{"key":"e_1_3_1_18_2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0092-8674(00)81146-0","volume":"93","author":"Nichols W. C.","year":"1998","unstructured":"Nichols W. C., et al., Cell 93, 61 (1998).","journal-title":"Cell"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80835-1"},{"key":"e_1_3_1_20_2","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/0955-0674(95)80010-7","volume":"7","author":"Pelham H. R.","year":"1995","unstructured":"Pelham H. R., Curr. Opin. Cell Biol. 7, 530 (1995).","journal-title":"Curr. Opin. Cell Biol."},{"key":"e_1_3_1_21_2","unstructured":"For data plots of the experiments in Figs. 2 and 3 see Science Online (www.sciencemag.org\/cgi\/content\/full\/294\/5546\/1528\/DC1)."},{"key":"e_1_3_1_22_2","unstructured":"Strain CBY966 (10) was transformed with pAC530 (12) to generate CBY1160 ( erv29 \u0394 ::KAN ERV29-HA ). FY834 (10) was transformed with pDA6300 ( Mf \u03b1 1-LEU2-2 \u03bc) to generate CBY1161. CBY1162 contains pRS306-ERV29 integrated into the URA3 locus of CBY1161."},{"key":"e_1_3_1_23_2","unstructured":"Antiserum to Erv29p was raised against a GST-Erv29p fusion protein. A 262\u2013base pair 5\u2032 Apo I fragment from ERV29 was inserted into pGEX-5x-3 and fusion protein was purified as described by the manufacturer (Amersham Pharmacia). Antiserum was produced in rabbits by Covance Inc. (Denver PA)."},{"key":"e_1_3_1_24_2","unstructured":"Cross-linking reactions (0.05 ml) were performed in buffer 88 [25 mM Hepes (pH 7.5) 150 mM KOAc 0.25 M sorbitol and 5 mM MgOAc] for 20 min at 20\u00b0C. Reactions were quenched with 10 mM glycine for 5 min at 20\u00b0C solubilized with an equal volume of 1% SDS and heat-denatured. Where indicated proteins were immunoprecipitated with antibodies after dilution with 1 ml of IP buffer [15 mM tris-Cl (pH 7.5) 150 mM NaCl and 1% Triton X-100]."},{"key":"e_1_3_1_25_2","unstructured":"We thank A. Cooper for providing the HA-tagged Erv29p construct R. Gilmore for antiserum to CPY and R. Schekman for antiserum to \u03b1f. Supported by grants from the National Institute of General Medical Sciences and the Pew Scholars Program in the Biomedical Sciences."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1065224","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T17:02:10Z","timestamp":1704819730000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1065224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11,16]]},"references-count":24,"journal-issue":{"issue":"5546","published-print":{"date-parts":[[2001,11,16]]}},"alternative-id":["10.1126\/science.1065224"],"URL":"https:\/\/doi.org\/10.1126\/science.1065224","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1002087.22054","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1002087.29109","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1002087.32204","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,11,16]]}}}