{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:08:18Z","timestamp":1792062498668,"version":"4.4.0"},"reference-count":26,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5549","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2001,12,7]]},"abstract":"<jats:p>\n                    The mechanisms controlling neural stem cell proliferation are poorly understood. Here we demonstrate that the PTEN tumor suppressor plays an important role in regulating neural stem\/progenitor cells in vivo and in vitro. Mice lacking PTEN exhibited enlarged, histoarchitecturally abnormal brains, which resulted from increased cell proliferation, decreased cell death, and enlarged cell size. Neurosphere cultures revealed a greater proliferation capacity for tripotent\n                    <jats:italic>Pten<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    central nervous system stem\/progenitor cells, which can be attributed, at least in part, to a shortened cell cycle. However, cell fate commitments of the progenitors were largely undisturbed. Our results suggest that PTEN negatively regulates neural stem cell proliferation.\n                  <\/jats:p>","DOI":"10.1126\/science.1065518","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:47:15Z","timestamp":1027748835000},"page":"2186-2189","source":"Crossref","is-referenced-by-count":700,"title":["Negative Regulation of Neural Stem\/Progenitor Cell Proliferation by the\n                    <i>Pten<\/i>\n                    Tumor Suppressor Gene in Vivo"],"prefix":"10.1126","volume":"294","author":[{"given":"Matthias","family":"Groszer","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute,"},{"name":"Department of Molecular and Medical Pharmacology,"},{"name":"Department of Pathology and Laboratory Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Erickson","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Pharmacology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deirdre D.","family":"Scripture-Adams","sequence":"additional","affiliation":[{"name":"Department of Medicine and AIDS Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Lesche","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute,"},{"name":"Department of Molecular and Medical Pharmacology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Trumpp","sequence":"additional","affiliation":[{"name":"Institut Suisse de Recherche Expe\u0301rimentale sur le Cancer, Ch. de Boveresses 155, 1066 Epalinges\/Lausanne, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome A.","family":"Zack","sequence":"additional","affiliation":[{"name":"Department of Medicine and AIDS Institute,"},{"name":"Department of Microbiology and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harley I.","family":"Kornblum","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Pharmacology,"},{"name":"Department of Pediatrics,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Pharmacology,"},{"name":"Department of Pathology and Laboratory Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Wu","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute,"},{"name":"Department of Molecular and Medical Pharmacology,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","unstructured":"J. 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Nature Genet. 25 55 (2000).","DOI":"10.1038\/75596"},{"key":"e_1_3_1_27_2","unstructured":"We thank K. Tatsukawa Y. Wang and D. Irvin for valuable technical assistance and D. van der Kooy S. Morrison H. Herschman Y. Sun G. Fan J. Yuan and members of our laboratories for helpful comments on the manuscript. H.W. is an Assistant Investigator of the Howard Hughes Medical Institute (HHMI). M.G. is supported by the Swiss National Science Foundation. R.L. was partially supported by HHMI and the Deutsche Forschungsgemeinschaft. Supported by NIH grants NS38489 (X.L.) and MH062800-01 (H.I.K.) and by U.S. Department of Defense grants PC991538 (H.W.) and A136059 (J.A.Z.)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1065518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T17:32:08Z","timestamp":1704821528000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1065518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12,7]]},"references-count":26,"journal-issue":{"issue":"5549","published-print":{"date-parts":[[2001,12,7]]}},"alternative-id":["10.1126\/science.1065518"],"URL":"https:\/\/doi.org\/10.1126\/science.1065518","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1002416.30570","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1002416.30204","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1002416.27805","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,12,7]]}}}