{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:12:53Z","timestamp":1792282373736,"version":"4.4.0"},"reference-count":22,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5559","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2002,2,22]]},"abstract":"<jats:p>A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration\u2013dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of \u223c 100,000:1.<\/jats:p>","DOI":"10.1126\/science.1067003","type":"journal-article","created":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T06:51:32Z","timestamp":1633330292000},"page":"1503-1506","source":"Crossref","is-referenced-by-count":1011,"title":["Array-Based Electrical Detection of DNA with Nanoparticle Probes"],"prefix":"10.1126","volume":"295","author":[{"given":"So-Jung","family":"Park","sequence":"first","affiliation":[{"name":"Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, IL 60208, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Andrew","family":"Taton","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, IL 60208, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad A.","family":"Mirkin","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, IL 60208, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","unstructured":"G. H. Keller M. M. Manak DNA Probes (Stocktonton NY 1989)."},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5329.1078"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja972332i"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5485.1757"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0102639"},{"key":"e_1_3_1_7_2","doi-asserted-by":"crossref","unstructured":"M. E. Napier et al. Bioconjugate Chem. 8 906 (1997).","DOI":"10.1021\/bc9701149"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.24.4830"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja010045f"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja001215b"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5385.2016"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.294.5540.137"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3773(20011001)40:19<3685::AID-ANIE3685>3.0.CO;2-E"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/la981729c"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3773(20001103)39:21<3845::AID-ANIE3845>3.0.CO;2-O"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/la0102408"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5324.393"},{"key":"e_1_3_1_18_2","doi-asserted-by":"crossref","unstructured":"L. A. Chrisey G. U. Lee C. E. O'Ferrall Nucleic Acids Res. 24 3031 (1996).","DOI":"10.1093\/nar\/24.15.3031"},{"key":"e_1_3_1_19_2","doi-asserted-by":"crossref","unstructured":"F. Eckstein Oligonucleotides and Analogues (Oxford Univ. Press New York 1991).","DOI":"10.1093\/oso\/9780199632800.001.0001"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.2.797"},{"key":"e_1_3_1_21_2","unstructured":"Supplementary data are available on Science Online at www.sciencemag.org\/cgi\/content\/full\/295\/5559\/1503\/DC1."},{"key":"e_1_3_1_22_2","unstructured":"The nanoparticle coverage on the ITO surface does not necessarily correspond exactly with the coverage on the SiO 2 surface. However the SEM images provide information about the silver-deposition process on an oxide surface modified with oligonucleotide-functionalized nanoparticles rather than a correlation between the amount of deposited silver and resistance values in Fig. 2."},{"key":"e_1_3_1_23_2","unstructured":"C.A.M. acknowledges the Air Force Office of Scientific Research (DURINT) and the Defense Advanced Research Projects Agency for support of this research."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1067003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T16:28:31Z","timestamp":1704817711000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1067003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2,22]]},"references-count":22,"journal-issue":{"issue":"5559","published-print":{"date-parts":[[2002,2,22]]}},"alternative-id":["10.1126\/science.1067003"],"URL":"https:\/\/doi.org\/10.1126\/science.1067003","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2,22]]}}}