{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:48:08Z","timestamp":1792622888128,"version":"4.5.0"},"reference-count":17,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5553","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2002,1,11]]},"abstract":"<jats:p>\n            Connexin genes encode intercellular channels that help to coordinate development. In mice, the targeted deletion of different connexins produces disparate effects on ocular growth and differentiation in the lens, and the need for multiple channel subunits is poorly understood. Knockout of\n            <jats:italic>Cx46<\/jats:italic>\n            causes a loss of homeostasis and cataracts. Deletion of\n            <jats:italic>Cx50<\/jats:italic>\n            results in reduced ocular growth and cataracts. Targeted replacement of\n            <jats:italic>Cx50<\/jats:italic>\n            with\n            <jats:italic>Cx46<\/jats:italic>\n            by genetic knock-in corrected defects in cellular differentiation and prevented cataracts, but did not restore normal growth. These data show that intrinsic properties of\n            <jats:italic>Cx50<\/jats:italic>\n            were required for cellular growth, whereas nonspecific restoration of communication by\n            <jats:italic>Cx46<\/jats:italic>\n            maintained differentiation.\n          <\/jats:p>","DOI":"10.1126\/science.1067582","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:47:15Z","timestamp":1027763235000},"page":"319-320","source":"Crossref","is-referenced-by-count":171,"title":["Unique and Redundant Connexin Contributions to Lens Development"],"prefix":"10.1126","volume":"295","author":[{"given":"Thomas W.","family":"White","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, NY 11794, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/S0960-9822(06)00302-2","volume":"7","author":"Temme A.","year":"1997","unstructured":"Temme A., et al., Curr. Biol. 7, 713 (1997).","journal-title":"Curr. Biol."},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/385525a0","volume":"385","author":"Simon A. M.","year":"1997","unstructured":"Simon A. M., Nature 385, 525 (1997).","journal-title":"Nature"},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"2808","DOI":"10.1074\/jbc.273.5.2808","volume":"273","author":"Bevans C. G.","year":"1998","unstructured":"Bevans C. G., J. Biol. Chem. 273, 2808 (1998).","journal-title":"J. Biol. Chem."},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1006\/dbio.2000.9638","volume":"220","author":"Beebe D. C.","year":"2000","unstructured":"Beebe D. C., Coats J. M., Dev. Biol. 220, 424 (2000).","journal-title":"Dev. Biol."},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0165-0173(99)00072-7","volume":"32","author":"White T. W.","year":"2000","unstructured":"White T. W., Bruzzone R., Brain Res. Rev. 32, 130 (2000).","journal-title":"Brain Res. Rev."},{"key":"e_1_3_1_7_2","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/S0092-8674(00)80471-7","volume":"91","author":"Gong X.","year":"1997","unstructured":"Gong X., et al., Cell 91, 833 (1997).","journal-title":"Cell"},{"key":"e_1_3_1_8_2","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1083\/jcb.143.3.815","volume":"143","author":"White T. W.","year":"1998","unstructured":"White T. W., J. Cell. Biol. 143, 815 (1998).","journal-title":"J. Cell. Biol."},{"key":"e_1_3_1_9_2","doi-asserted-by":"crossref","first-page":"15303","DOI":"10.1073\/pnas.95.26.15303","volume":"95","author":"Gong X.","year":"1998","unstructured":"Gong X., Proc. Natl. Acad. Sci. U.S.A. 95, 15303 (1998).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"e_1_3_1_10_2","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1111\/j.1469-7793.1999.0673s.x","volume":"517","author":"Srinivas M.","year":"1999","unstructured":"Srinivas M., et al., J. Physiol. 517, 673 (1999).","journal-title":"J. Physiol."},{"key":"e_1_3_1_11_2","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1016\/S0006-3495(00)76444-7","volume":"79","author":"Hopperstad M. G.","year":"2000","unstructured":"Hopperstad M. G., Biophys. J. 79, 1954 (2000).","journal-title":"Biophys. J."},{"key":"e_1_3_1_12_2","unstructured":"Cx50 was replaced with Cx46 by standard PCR techniques. A floxed pgk-neomycin resistance cassette was placed within an intron in the 5\u2032 untranslated region of Cx50. A pgk-diphtheria toxin A chain cassette was placed outside the 5\u2032 homology region. Homologous recombinants were identified by PCR screening at a frequency of 1 in 17 neo r colonies. Two independent Cx50KI46 embryonic stem cell clones were transiently transfected with Cre recombinase to excise the pkg-NEO marker leaving one 34\u2013base pair loxP site within the intron. Both Cx50KI46 embryonic stem cell clones were injected into C57BL\/6 blastocysts and produced founders exhibiting germ-line transmission. Cx50KI46 mice were maintained in the 129\/SvEv genetic background. Analytical methods and antisera used were as described (7)."},{"key":"e_1_3_1_13_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1046\/j.1432-0436.1996.6120077.x","volume":"61","author":"Wride M. A.","year":"1996","unstructured":"Wride M. A., Differentiation 61, 77 (1996).","journal-title":"Differentiation"},{"key":"e_1_3_1_14_2","unstructured":"T. W. White data not shown."},{"key":"e_1_3_1_15_2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1432-0436.1981.tb01141.x","volume":"19","author":"Piatigorsky J.","year":"1981","unstructured":"Piatigorsky J., Differentiation 19, 134 (1981).","journal-title":"Differentiation"},{"key":"e_1_3_1_16_2","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1016\/S0960-9822(00)00690-4","volume":"10","author":"Plum A.","year":"2000","unstructured":"Plum A., et al., Curr. Biol. 10, 1083 (2000).","journal-title":"Curr. Biol."},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0001q.x"},{"key":"e_1_3_1_18_2","unstructured":"I thank C. Sellitto for assistance. Supported by NIH grants EY13163 EY02430 and GM37751 (T.W.W. D. A. Goodenough and D. L. Paul)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1067582","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T22:48:06Z","timestamp":1704840486000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1067582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1,11]]},"references-count":17,"journal-issue":{"issue":"5553","published-print":{"date-parts":[[2002,1,11]]}},"alternative-id":["10.1126\/science.1067582"],"URL":"https:\/\/doi.org\/10.1126\/science.1067582","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1,11]]}}}