{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:23:51Z","timestamp":1791350631603,"version":"4.3.1"},"reference-count":23,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5568","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2002,4,26]]},"abstract":"<jats:p>\n                    Sur2 is a metazoan Mediator subunit that interacts with the adenovirus E1A protein and functions in a mitogen-activated protein kinase pathway required for vulva development in\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    . We generated\n                    <jats:italic>sur2<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    embryonic stem cells to analyze its function as a mammalian Mediator component. Our results show that Sur2 forms a subcomplex of the Mediator with two other subunits, TRAP\/Med100 and 95. Knock-out of Sur2 prevents activation by E1A-CR3 and the mitogen-activated protein kinase\u2013regulated ETS transcription factor Elk-1, but not by multiple other transcription factors. These results imply that specific activation domains stimulate transcription by binding to distinct Mediator subunits. Activation by E1A and Elk-1 requires recruitment of Mediator to a promoter by binding to its Sur2 subunit.\n                  <\/jats:p>","DOI":"10.1126\/science.1068943","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:54:32Z","timestamp":1027749272000},"page":"755-758","source":"Crossref","is-referenced-by-count":221,"title":["Transcription Control by E1A and MAP Kinase Pathway via Sur2 Mediator Subunit"],"prefix":"10.1126","volume":"296","author":[{"given":"Jennitte L.","family":"Stevens","sequence":"first","affiliation":[{"name":"Molecular Biology Institute, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg T.","family":"Cantin","sequence":"additional","affiliation":[{"name":"Molecular Biology Institute, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Wang","sequence":"additional","affiliation":[{"name":"Molecular Biology Institute, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrej","family":"Shevchenko","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Shevchenko","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold J.","family":"Berk","sequence":"additional","affiliation":[{"name":"Molecular Biology Institute, University of California, Los Angeles, 611 Charles Young Drive East, Los Angeles, CA 90095, USA."},{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.34.1.77"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.729"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)01596-6"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(00)00209-X"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/20466"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.18.2251"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01211-0"},{"key":"e_1_3_1_9_2","unstructured":"Supplementary figures and details of experimental procedures are available on Science Online at www.sciencemag.org\/cgi\/content\/full\/1068943\/DC1"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.14.4604-4613.2001"},{"key":"e_1_3_1_11_2","unstructured":"G. Wang A. J. Berk unpublished data."},{"key":"e_1_3_1_12_2","unstructured":"R. G. Roeder personal communication."},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90045-X"},{"key":"e_1_3_1_14_2","unstructured":"J. L. Stevens A. J. Berk unpublished data."},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm715"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82309-2"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200839"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.2.1086"},{"key":"e_1_3_1_19_2","doi-asserted-by":"crossref","unstructured":"R. Graham M. Gilman Science 251 189 (1991).","DOI":"10.1126\/science.1898992"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102678200"},{"key":"e_1_3_1_21_2","first-page":"1","volume":"1242","author":"Johansen F.-E.","year":"1995","unstructured":"Johansen F.-E., Prywes R., Biochim. Biophys. Acta 1242, 1 (1995).","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(99)80021-5"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80247-6"},{"key":"e_1_3_1_24_2","unstructured":"Supported by USPHS NIH grant CA25235. J.L.S. and G.T.C. partially supported by USPHS NRSA GM07185. We thank K. Lyons S. Joseph X. Liu and H. Wu for assistance with ES cell work; L. Freedman E. Lees D. Reinberg and R. Roeder for antibodies; and C. Eng and Z. Zhou for technical assistance."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1068943","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T22:37:00Z","timestamp":1704839820000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1068943"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4,26]]},"references-count":23,"journal-issue":{"issue":"5568","published-print":{"date-parts":[[2002,4,26]]}},"alternative-id":["10.1126\/science.1068943"],"URL":"https:\/\/doi.org\/10.1126\/science.1068943","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1006404.79861","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1006404.85356","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4,26]]}}}